Nakagawa K, Kanno H, Miura Y
Radio Isotope Research Center, Department of Pediatrics, Fukushima Medical College, 1 Hikarigaoka, Fukushima-shi, 960-12, Japan.
Anal Biochem. 1997 Dec 1;254(1):31-5. doi: 10.1006/abio.1997.2372.
We have detected and analyzed a free radical in human cerebrospinal fluid (CSF) of acute lymphoblastic leukemia (ALL) for the first time using electron paramagnetic resonance (EPR) at ambient temperature. We have also introduced an alternative capillary method to measure the radical. EPR spectra of the radical show a characteristic doublet with hyperfine coupling value of 1.8 G and g = 2.005. Based on EPR measurements, computer simulation, and literature values, we have determined that the species is ascorbyl radical (AsR). The radical has been investigated in CSF samples from ALL patients having no therapy, undergoing chemotherapy, and following therapy. Determination of the ascorbyl radical concentrations in CSF and serum was attempted using known concentrations of a nitroxyl radical. In addition, comparison in CSF and serum for ALL has been made along with statistical analyses of the data obtained. We found that AsR in CSF and serum has a strong correlation in patients undergoing chemotherapy (n = 57, r = 0.57, P < 0.0001). Ascorbate in CSF and serum show good correlation in patients having therapy but not for patients after therapy.
我们首次在常温下使用电子顺磁共振（EPR）检测并分析了急性淋巴细胞白血病（ALL）患者脑脊液（CSF）中的一种自由基。我们还引入了一种替代的毛细管方法来测量该自由基。该自由基的EPR谱显示出特征性的双峰，超精细耦合值为1.8 G，g = 2.005。基于EPR测量、计算机模拟和文献值，我们确定该物质为抗坏血酸自由基（AsR）。我们对未接受治疗、正在接受化疗以及化疗后的ALL患者的脑脊液样本中的该自由基进行了研究。尝试使用已知浓度的硝酰自由基来测定脑脊液和血清中的抗坏血酸自由基浓度。此外，还对ALL患者的脑脊液和血清进行了比较，并对所得数据进行了统计分析。我们发现，正在接受化疗的患者（n = 57，r = 0.57，P < 0.0001）脑脊液和血清中的AsR具有很强的相关性。接受治疗的患者脑脊液和血清中的抗坏血酸盐显示出良好的相关性，但治疗后的患者则不然。
