人胆小管多特异性有机阴离子转运体（cMOAT）在人肺癌、胃癌和结肠癌细胞中表达。

Human canalicular multispecific organic anion transporter (cMOAT) is expressed in human lung, gastric, and colorectal cancer cells.

作者信息

Narasaki F, Oka M, Nakano R, Ikeda K, Fukuda M, Nakamura T, Soda H, Nakagawa M, Kuwano M, Kohno S

机构信息

Second Department of Internal Medicine, Nagasaki University School of Medicine, Japan.

出版信息

Biochem Biophys Res Commun. 1997 Nov 26;240(3):606-11. doi: 10.1006/bbrc.1997.7703.

DOI:10.1006/bbrc.1997.7703

PMID:9398612

Abstract

Human canalicular multispecific organic anion transporter (cMOAT), a glutathione conjugate membrane transporter, has been isolated from cisplatin-resistant cancer cells and is distributed mainly in normal liver. We analyzed the expression of human cMOAT in 14 lung, 11 gastric, and 9 colorectal non-drug-selected human cancer cells, two multidrug-resistant cells, and one cisplatin-resistant cells, using quantitative RT-PCR and newly developed anti-human cMOAT antibody. All cell lines analyzed here expressed human cMOAT at the level of mRNA and protein, and some of them expressed higher levels of human cMOAT than the cisplatin-resistant cells. The two multidrug-resistant cell lines co-expressed human cMOAT gene and both or either of MRP and MDR1 genes. Immunostaining showed that human cMOAT was predominantly localized to the cytoplasm of these single cells. Our results indicate that human cMOAT is expressed in various human cancer cells including drug-resistant cells.

摘要

人胆小管多特异性有机阴离子转运体（cMOAT）是一种谷胱甘肽共轭膜转运体，已从顺铂耐药癌细胞中分离出来，主要分布于正常肝脏。我们使用定量逆转录聚合酶链反应（RT-PCR）和新开发的抗人cMOAT抗体，分析了14种未经过药物筛选的人肺癌细胞、11种胃癌细胞、9种结肠癌细胞、两种多药耐药细胞和一种顺铂耐药细胞中人cMOAT的表达情况。这里分析的所有细胞系在mRNA和蛋白质水平均表达人cMOAT，其中一些细胞系表达的人cMOAT水平高于顺铂耐药细胞。两种多药耐药细胞系共同表达人cMOAT基因以及MRP和MDR1基因中的一个或两个。免疫染色显示，人cMOAT主要定位于这些单细胞的细胞质中。我们的结果表明，人cMOAT在包括耐药细胞在内的各种人癌细胞中均有表达。