Fauconnet V, Pouliquen H, Pinault L
Ecole Nationale Vétérinaire de Nantes, Laboratoire de Pharmacie et Toxicologie, Nantes, France.
J Anal Toxicol. 1997 Nov-Dec;21(7):548-53. doi: 10.1093/jat/21.7.548.
A reversed-phase high-performance liquid chromatographic method was developed for the analysis of eight anticoagulant rodenticides in animal liver. Coumarinic anticoagulant rodenticides (brodifacoum, bromadiolone, coumachlor, coumatetralyl, difenacoum, and warfarin) were detected by using a gradient elution and a fluorimetric detection. Indanedione anticoagulant rodenticides (chlorophacinone and diphacinone) were detected by using an isocratic elution and an UV detection. Anticoagulants were extracted from liver with mixtures of acetone/diethylether and acetone/chloroform. Extracts were applied to solid-phase extraction cartridges. Linearity was checked over the concentration range 0.1-0.6 microgram/g. Relative standard deviations of within-run and between-run variability were all between 5.7 and 10.3%. Recoveries from spiked liver samples were between 51.7 (difenacoum) and 78.2% (warfarin). Limits of detection were between 0.01 (difenacoum and warfarin) and 0.11 microgram/g (chlorophacinone).
建立了一种反相高效液相色谱法，用于分析动物肝脏中的八种抗凝血灭鼠剂。香豆素类抗凝血灭鼠剂（溴敌隆、溴鼠灵、杀鼠氯、杀鼠迷、敌鼠和华法林）采用梯度洗脱和荧光检测进行测定。茚满二酮类抗凝血灭鼠剂（氯鼠酮和敌鼠酮）采用等度洗脱和紫外检测进行测定。抗凝血剂用丙酮/乙醚和丙酮/氯仿的混合物从肝脏中提取。提取物应用于固相萃取柱。在0.1 - 0.6微克/克的浓度范围内检查线性。批内和批间变异的相对标准偏差均在5.7%至10.3%之间。加标肝脏样品的回收率在51.7%（敌鼠）至78.2%（华法林）之间。检测限在0.01（敌鼠和华法林）至0.11微克/克（氯鼠酮）之间。
