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低频超声能量在嗜菌新圆线虫中诱导可见突变

Visible mutation induction in Mormoniella by low frequency ultrasonic energy.

作者信息

Grubbs S C, Conner G W

出版信息

J Hered. 1976 May-Jun;67(3):191-3. doi: 10.1093/oxfordjournals.jhered.a108706.

DOI:10.1093/oxfordjournals.jhered.a108706
PMID:939919
Abstract

Low frequency ultrasonic energy was utilized in an attempt to induce visible mutations in the parasitoid wasp Mormoniella vitripennis (Walker). Ultrasonic exposure at a frequency of 20,000 cycles per second was accomplished in aqueous medium with a commercially obtained energy source. Among the 22,396 progeny of exposed males and females, 63 phenotypically changed wasps were recovered. Three of these changes from exposed males and two from exposed females proved to be genetically transmissible. No transmissible changes were found among 4,739 control progeny. This study demonstrates that low frequency ultrasound may be used as an effective mutagenic agent with this organism, and suggests that it may have applications with other genetic systems.

摘要

研究人员利用低频超声能量试图在寄生蜂米氏啮小蜂(Walker)中诱导出可见的突变。使用市售能量源在水介质中以每秒20,000次循环的频率进行超声照射。在暴露的雄性和雌性后代的22,396只子代中,回收了63只表型发生变化的黄蜂。其中,来自暴露雄性的3个变化和来自暴露雌性的2个变化被证明是可遗传的。在4,739只对照子代中未发现可遗传的变化。这项研究表明,低频超声可作为这种生物的一种有效的诱变剂,并表明它可能在其他遗传系统中具有应用价值。

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