Hammond R W, Kogel R, Ramirez P
Molecular Plant Pathology Laboratory, U.S. Department of Agriculture, Agricultural Research Service, Beltsville, Maryland 20705, USA.
J Gen Virol. 1997 Dec;78 ( Pt 12):3153-9. doi: 10.1099/0022-1317-78-12-3153.
We have examined the molecular epidemiology of the leafhopper-borne maize rayado fino virus (MRFV) in Latin America. The coat protein gene and 3' non-translated region of 14 isolates of MRFV collected from Latin America and the United States were sequenced and phylogenetic relationships examined. The nucleotide sequence revealed remarkable conservation, with a sequence similarity of 88-99%. Phylogenetic analysis of sequence data obtained from a 633 bp fragment showed that MRFV has diverged into three main clusters, i.e. the geographically distinct northern and southern isolates and the Colombian isolates. Significant differences between the isolates collected from Colombia, previously named maize rayado colombiana virus, based upon differences in symptomatology and serological relationships to MRFV, and the other MRFV isolates, provides additional evidence supporting its designation as a unique strain of MRFV.
我们研究了拉丁美洲叶蝉传播的玉米雷亚多菲诺病毒（MRFV）的分子流行病学。对从拉丁美洲和美国收集的14株MRFV分离株的外壳蛋白基因和3'非翻译区进行了测序，并检查了系统发育关系。核苷酸序列显示出显著的保守性，序列相似性为88-99%。对从633 bp片段获得的序列数据进行的系统发育分析表明，MRFV已分化为三个主要簇，即地理上不同的北部和南部分离株以及哥伦比亚分离株。根据症状学差异以及与MRFV的血清学关系，从哥伦比亚收集的分离株（以前称为玉米雷亚多哥伦比亚病毒）与其他MRFV分离株之间存在显著差异，这为支持将其指定为MRFV的独特毒株提供了额外证据。
