Hatta Y, Spirin K, Tasaka T, Morosetti R, Said J W, Yamada Y, Tomonaga M, Koeffler H P
Division of Hematology/Oncology, Cedars-Sinai Research Institute, UCLA School of Medicine, Los Angeles, California 90048, USA.
Br J Haematol. 1997 Dec;99(3):665-7. doi: 10.1046/j.1365-2141.1997.4333243.x.
p18INK4C, a cyclin-dependent kinase inhibitor, is a homologue of p15INK4B and p16INK4A which are frequently altered in a variety of malignancies. We searched for structural alterations of the p18INK4C gene in 44 adult T-cell leukaemias (ATLs), 101 non-Hodgkin's lymphomas (NHLs), two polyclonal B-cell proliferations, seven ATL cell lines and seven leukaemia/lymphoma cell lines, by Southern blot and polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analyses. No genomic alterations of the p18INK4C gene were found in any of the samples. By RT-PCR, p18INK4C was not expressed in three of five ATL cell lines, whereas it was expressed in all the non-ATL leukaemia/lymphoma cell lines. Tax did not inhibit the expression of p18INK4C in tax-expressing Jurkat cells.
p18INK4C是一种细胞周期蛋白依赖性激酶抑制剂，是p15INK4B和p16INK4A的同源物，它们在多种恶性肿瘤中经常发生改变。我们通过Southern印迹和聚合酶链反应-单链构象多态性（PCR-SSCP）分析，在44例成人T细胞白血病（ATL）、101例非霍奇金淋巴瘤（NHL）、2例多克隆B细胞增殖、7株ATL细胞系和7株白血病/淋巴瘤细胞系中寻找p18INK4C基因的结构改变。在任何样本中均未发现p18INK4C基因的基因组改变。通过RT-PCR，p18INK4C在5株ATL细胞系中的3株中未表达，而在所有非ATL白血病/淋巴瘤细胞系中均有表达。Tax在表达tax的Jurkat细胞中不抑制p18INK4C的表达。
