CD34阳性急性白血病中Thy-1表达的流式细胞术分析

Flow cytometric analysis of Thy-1 expression in CD34-positive acute leukemia.

作者信息

Inaba T, Shimazaki C, Sumikuma T, Takahashi R, Hirai H, Kikuta T, Sudo Y, Yamagata N, Ashihara E, Goto H, Fujita N, Yoshimura M, Nakagawa M

机构信息

Department of Clinical Laboratory and Medicine, Kyoto Prefectural University of Medicine, Japan.

出版信息

Int J Hematol. 1997 Oct;66(3):315-23. doi: 10.1016/s0925-5710(97)00051-0.

DOI:10.1016/s0925-5710(97)00051-0

PMID:9401277

Abstract

We analyzed the expression of the Thy-1 antigen (CD90) in CD34+ acute leukemia using two-color flow cytometry. Leukemic cells were obtained from the bone marrow (BM) and/or the peripheral blood (PB) of 57 patients: 37 with acute myelogenous leukemia (AML) including nine with secondary AML following myelodysplastic syndrome (MDS/AML), and 20 with acute lymphoblastic leukemia (ALL) including three with chronic myelogenous leukemia in blast crisis (CML-BC) of the lymphoid type. Among these patients, one (3.6%) with de novo AML, two (22.2%) with MDS/AML, three (17.6%) with de novo ALL, and two (66.7%) with CML-BC coexpressed CD34 and Thy-1 (CD34+ Thy-1+) on more than 20% of the mononuclear cells within 'lymph' plus 'blast' window. Thy-1 was rarely expressed in de novo acute leukemia especially in AML. Interestingly, in 1 patient with CML-BC, the leukemic cells in BM were divided into two subpopulations (CD34+ Thy-1low and CD34+ Thy-1high), whereas most of the CD34+ leukemic cells in PB were Thy-1high. However, the mechanism for the mobilization of CD34+ Thy-1high leukemic cells into the PB is unknown.

摘要

我们采用双色流式细胞术分析了CD34⁺急性白血病中Thy-1抗原（CD90）的表达情况。白血病细胞取自57例患者的骨髓（BM）和/或外周血（PB）：37例急性髓系白血病（AML）患者，其中9例为骨髓增生异常综合征继发的急性髓系白血病（MDS/AML）；20例急性淋巴细胞白血病（ALL）患者，其中3例为淋巴系慢性髓系白血病急变期（CML-BC）。在这些患者中，1例（3.6%）初发AML、2例（22.2%）MDS/AML、3例（17.6%）初发ALL以及2例（66.7%）CML-BC患者的“淋巴细胞”加“原始细胞”窗内超过20%的单核细胞共表达CD34和Thy-1（CD34⁺Thy-1⁺）。Thy-1在初发急性白血病中很少表达，尤其是在AML中。有趣的是，在1例CML-BC患者中，骨髓中的白血病细胞分为两个亚群（CD34⁺Thy-1低和CD34⁺Thy-1高），而外周血中大多数CD34⁺白血病细胞为Thy-1高。然而，CD34⁺Thy-1高白血病细胞动员至外周血的机制尚不清楚。