Thür B, Hilbe M, Strasser M, Ehrensperger F
Institute of Veterinary Pathology, University of Zurich, Switzerland.
Am J Vet Res. 1997 Dec;58(12):1371-5.
To establish a reliable, rapid, economical method for detection of pestivirus infection in bovine and ovine fetuses and to examine participation of these viruses in abortions and neonatal mortality.
213 bovine and 31 ovine fetuses, as well as 36 newborn calves and 25 lambs, which had died within 3 days after birth, were tested for bovine viral diarrhea virus (BVDV) and border disease virus by use of different methods.
Detection of BVDV in fetuses was performed by immunohistochemical methods, using a panel of monoclonal antibodies against pestivirus antigens on cryostat and paraffin sections and by virus isolation in cell culture; in some instances, an antigencapture ELISA was performed. Results of the various methods were compared.
Sensitivity of BVDV detection by immunohistochemical methods and virus isolation in cell culture was equal; however, it decreased in association with autolysis. In autolytic fetuses, use of formalin-fixed, paraffin-embedded brain sections was the most favorable method. Antigen detection by ELISA was less sensitive.
Immunohistochemical analysis of cryostat sections of brain, skin, thyroid gland, abomasum, and placenta is a rapid, sensitive method for detecting pestiviruses in fetuses. In the presence of advanced autolysis, this method used on formalin-fixed, paraffin-embedded brain sections is recommended over the other described methods.
建立一种可靠、快速且经济的方法来检测牛和羊胎儿中的瘟病毒感染，并研究这些病毒在流产和新生儿死亡中的作用。
对213个牛胎儿、31个羊胎儿以及36头出生后3天内死亡的新生犊牛和25只出生后3天内死亡的羔羊，采用不同方法检测牛病毒性腹泻病毒（BVDV）和边界病病毒。
通过免疫组化方法检测胎儿中的BVDV，使用一组针对瘟病毒抗原的单克隆抗体对冷冻切片和石蜡切片进行检测，并通过细胞培养进行病毒分离；在某些情况下，进行抗原捕获ELISA检测。比较各种方法的结果。
免疫组化方法和细胞培养病毒分离检测BVDV的灵敏度相同；然而，随着自溶的发生，灵敏度会降低。在自溶胎儿中，使用福尔马林固定、石蜡包埋的脑切片是最有利的方法。ELISA抗原检测灵敏度较低。
对脑、皮肤、甲状腺、皱胃和胎盘的冷冻切片进行免疫组化分析是检测胎儿中瘟病毒的快速、灵敏方法。在存在严重自溶的情况下，推荐对福尔马林固定、石蜡包埋的脑切片使用该方法，而不是其他所述方法。
