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土耳其北部省份流产胎儿中瘟病毒的分子检测

Molecular detection of pestiviruses in aborted foetuses from provinces in northern Turkey.

作者信息

Albayrak Harun, Gumusova Semra Okur, Ozan Emre, Yazici Zafer

机构信息

Department of Virology, Faculty of Veterinary Medicine, Ondokuz Mayis University, 55139 Atakum, Samsun, Turkey.

出版信息

Trop Anim Health Prod. 2012 Apr;44(4):677-80. doi: 10.1007/s11250-011-9955-5. Epub 2011 Aug 14.

Abstract

All pestiviruses are important veterinary pathogens causing economic losses in cattle, sheep and pigs. Besides the important economical losses, pestiviruses may compromise the normal immune response to other pathogens and increase the severity of other infections in sheep. In this study, aborted foetuses (cattle and sheep) in either coastal or inland Black Sea region of Turkey were surveyed for the presence of RNA from pestiviruses (bovine viral diarrhoea virus (BVDV), border disease virus (BDV)). The presence of BVDV RNA was found in 6 of 21 aborted calves (28.57%), although BDV RNA was detected in 14 of 21 aborted lambs (66.66%) by reverse transcriptase polymerase chain reaction. This study also investigates the distribution of viral RNA within the brain, liver and lung of aborted foetuses. The viral RNA positivity rates for the organs varied and were as follows: brain 40.47% and liver and lung 38.09%. The results revealed that pestiviruses are important abort pathogen in the provinces of northern Turkey.

摘要

所有瘟病毒都是重要的兽医病原体,会给牛、羊和猪带来经济损失。除了造成重大经济损失外,瘟病毒可能会损害对其他病原体的正常免疫反应,并加重绵羊其他感染的严重程度。在本研究中,对土耳其黑海沿岸或内陆地区的流产胎儿(牛和羊)进行了调查,以检测瘟病毒(牛病毒性腹泻病毒(BVDV)、边界病病毒(BDV))的RNA。通过逆转录聚合酶链反应,在21头流产小牛中有6头(28.57%)检测到BVDV RNA,而在21只流产羔羊中有14只(66.66%)检测到BDV RNA。本研究还调查了流产胎儿脑、肝和肺内病毒RNA的分布情况。各器官的病毒RNA阳性率各不相同,具体如下:脑为40.47%,肝和肺为38.09%。结果表明,瘟病毒是土耳其北部省份重要的流产病原体。

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