硫醇化合物对粘质沙雷氏菌产生的金属β-内酰胺酶的抑制作用。
Inhibition of the metallo-beta-lactamase produced from Serratia marcescens by thiol compounds.
作者信息
Goto M, Takahashi T, Yamashita F, Koreeda A, Mori H, Ohta M, Arakawa Y
机构信息
Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
出版信息
Biol Pharm Bull. 1997 Nov;20(11):1136-40. doi: 10.1248/bpb.20.1136.
Low molecular weight thiol compounds have been found to be strong inhibitors of metallo-beta-lactamase (IMP-1) produced by Serratia marcescens TN9106, which was expressed by Echerichia coli JM109 cells. Mercaptoacetic acid and 2-mercaptopropionic acid strongly and competitively inhibited IMP-1 with Ki of 0.23 and 0.19 microM, respectively. 2-Mercaptoethanol reversibly inhibited IMP-1 but did not show simple competitive inhibition.
已发现低分子量硫醇化合物是粘质沙雷氏菌TN9106产生的金属β-内酰胺酶（IMP-1）的强效抑制剂，该酶由大肠杆菌JM109细胞表达。巯基乙酸和2-巯基丙酸对IMP-1有强烈的竞争性抑制作用，其抑制常数（Ki）分别为0.23和0.19微摩尔。2-巯基乙醇对IMP-1有可逆性抑制作用，但未表现出简单的竞争性抑制。
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