通过定量微透析法测量未麻醉大鼠纹状体中的细胞外葡萄糖周转率。
Extracellular glucose turnover in the striatum of unanaesthetized rats measured by quantitative microdialysis.
作者信息
Fray A E, Boutelle M, Fillenz M
机构信息
University Laboratory of Physiology, Oxford, UK.
出版信息
J Physiol. 1997 Nov 1;504 ( Pt 3)(Pt 3):721-6. doi: 10.1111/j.1469-7793.1997.721bd.x.
Abstract
- Steady-state and time-resolved quantitative microdialysis was used to measure dialysate concentration, extracellular concentration and the in vivo recovery of glucose in rat striatum. 2. The extracellular concentration of glucose, determined by the zero net flux method of Lönnroth, was 350 +/- 20 microM and the in vivo recovery was 39 +/- 2%. 3. Veratridine caused a steep decrease in dialysate glucose after an initial delay of 7.5 min. When steady-state glucose levels had been reached in the presence of veratridine the extracellular concentration was reduced to zero, but there was no significant change in in vivo recovery. 4. Measurement of the dynamic changes during the administration of veratridine showed an immediate decrease in extracellular glucose concentration and a steep rise in in vivo recovery, which accounted for the delay in the delay in the decrease in dialysate glucose. When extracellular concentration reached zero, in vivo recovery returned to control levels.
摘要
- 采用稳态和时间分辨定量微透析法测量大鼠纹状体中透析液葡萄糖浓度、细胞外葡萄糖浓度及体内回收率。2. 采用Lönnroth的零净通量法测定，葡萄糖的细胞外浓度为350±20微摩尔，体内回收率为39±2%。3. 藜芦碱在初始延迟7.5分钟后导致透析液葡萄糖急剧下降。当在藜芦碱存在下达到稳态葡萄糖水平时，细胞外浓度降至零，但体内回收率无显著变化。4. 藜芦碱给药期间动态变化的测量显示，细胞外葡萄糖浓度立即下降，体内回收率急剧上升，这解释了透析液葡萄糖下降延迟的原因。当细胞外浓度降至零时，体内回收率恢复到对照水平。
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