Cha S K, Kim N H, Lee S M, Baik C S, Lee H T, Chung K S
Animal Resources Research Center, Kon-Kuk University, Seoul, Korea.
Reprod Fertil Dev. 1997;9(4):441-6. doi: 10.1071/r96078.
Activation rate, chromosome constituent and developmental pattern of porcine oocytes was examined in the presence and absence of cytochalasin B and cycloheximide following parthenogenetic stimulation. Treatment with cycloheximide after ethanol or Ca2+ ionophore treatment increased the incidence of activation. The percentage of oocytes with two or more female pronuclei was higher (P < 0.05) in oocytes treated with cytochalasin B than in control or cycloheximide-treated oocytes. Treatment with both electrical stimulation and cytochalasin B increased the incidence of diploid chromosome spreads, and accelerated development to the morula and blastocyst stage compared with the control and cycloheximide-treated groups, suggesting a role of ploidy in the development of parthenote.
孤雌激活刺激后，在有和没有细胞松弛素B及放线菌酮的情况下，对猪卵母细胞的激活率、染色体组成和发育模式进行了检测。乙醇或钙离子载体处理后用放线菌酮处理可提高激活发生率。用细胞松弛素B处理的卵母细胞中具有两个或更多雌性原核的卵母细胞百分比高于对照或放线菌酮处理的卵母细胞（P<0.05）。与对照组和放线菌酮处理组相比，电刺激和细胞松弛素B联合处理可提高二倍体染色体铺展的发生率，并加速发育至桑椹胚和囊胚阶段，提示倍性在孤雌胚发育中起作用。
