采用一种简单的非同位素筛选方法，对英国非胰岛素依赖型糖尿病同胞对群体中的一个主要易感基因座（D2S125）进行遗传连锁研究。

Genetic linkage study of a major susceptibility locus (D2S125) in a British population of non-insulin dependent diabetic sib-pairs using a simple non-isotopic screening method.

作者信息

Thomas A W, Sherratt E J, Gagg J W, Davies S A, Majid A, Alcolado J C

机构信息

University of Wales College of Medicine, Department of Medicine, Heath Park, Cardiff, UK.

出版信息

Hum Genet. 1997 Dec;101(2):212-3. doi: 10.1007/s004390050617.

DOI:10.1007/s004390050617

PMID:9402972

Abstract

A polymorphic microsatellite marker (D2S125) was recently reported to show significant linkage to non-insulin dependent diabetes mellitus (NIDDM) in a population of Mexican-American affected sib-pairs. We have used a simple non-isotopic screening technique employing the polymerase chain reaction (PCR) with a biotinylated primer to study the genetic linkage and allele frequency distribution of the D2S125 marker in a population of 109 British NIDDMs (62 possible affected sib-pairs). The analysis provided no evidence for linkage of the D2S125 marker in the British subjects (MLS = 0.029, P > 0.05). The PCR screening method used proved to be a convenient and reliable alternative to the radiolabelling of PCR products.

摘要

最近有报道称，一种多态性微卫星标记（D2S125）在一组患非胰岛素依赖型糖尿病（NIDDM）的墨西哥裔美国人同胞对群体中显示出与该病有显著连锁关系。我们采用了一种简单的非同位素筛选技术，该技术利用聚合酶链反应（PCR）和生物素化引物，来研究D2S125标记在109名英国NIDDM患者群体（62对可能的患病同胞对）中的遗传连锁和等位基因频率分布。分析结果未提供该D2S125标记在英国受试者中存在连锁关系的证据（对数优势比分（MLS）= 0.029，P > 0.05）。事实证明，所采用的PCR筛选方法是对PCR产物进行放射性标记的一种方便且可靠的替代方法。