Onozuka K, Kirikae T, Kirikae F, Suda Y, Kusumoto S, Yamamoto S, Shimamura T, Nakano M
Department of Microbiology, Jichi Medical School, Tochigi, Japan.
Microbiol Immunol. 1997;41(10):765-72. doi: 10.1111/j.1348-0421.1997.tb01924.x.
The role of CD14 in the phagocytosis and killing of microorganisms was investigated using macrophage-like cell lines, CD14-positive J774.1 cells and CD14-negative mutant J7.DEF.3 cells derived from J744.1 cells. The cells were infected with Salmonella typhimurium organisms of the smooth (S)-form LT2, mutant rough (R)-form TV148 or Staphylococcus aureus 248betaH. At 30 or 180 min incubation, the cells were washed and disrupted. Colony-forming units (CFUs) liberated from the disrupted cells were determined by quantitative cultivation, and the phagocytic index and killing rate were calculated. Both the phagocytic index and killing rate of J774.1 cells against LT2 organisms were greater than those of J7.DEF.3 cells. However, the index and rate of J774.1 cells against TV148 and 248betaH organisms were similar to those of the J7.DEF.3 cells. The phagocytosis of LT2 organisms by J774.1 cells was partially inhibited by S-form LPS (S-LPS) and anti-CD14 antibody, but not by R-chemotype LPS (R-LPS). These results suggest that CD14 participates in the phagocytosis of S-form Salmonella.
利用巨噬细胞样细胞系、源自J744.1细胞的CD14阳性J774.1细胞和CD14阴性突变体J7.DEF.3细胞，研究了CD14在吞噬和杀灭微生物中的作用。用光滑（S）型LT2、突变粗糙（R）型TV148或金黄色葡萄球菌248betaH的鼠伤寒沙门氏菌感染这些细胞。在孵育30或180分钟后，洗涤并裂解细胞。通过定量培养确定从裂解细胞中释放的菌落形成单位（CFU），并计算吞噬指数和杀灭率。J774.1细胞对LT2菌的吞噬指数和杀灭率均高于J7.DEF.3细胞。然而，J774.1细胞对TV148和248betaH菌的指数和比率与J7.DEF.3细胞相似。J774.1细胞对LT2菌的吞噬作用部分受到S型脂多糖（S-LPS）和抗CD14抗体的抑制，但不受R化学型脂多糖（R-LPS）的抑制。这些结果表明CD14参与S型沙门氏菌的吞噬作用。
