Sato M, Suzuki Y, Okuda T, Yokotsuka K
Wines & Spirits Research Center, Mercian Corporation, Fujisawa, Japan.
Biosci Biotechnol Biochem. 1997 Nov;61(11):1800-5. doi: 10.1271/bbb.61.1800.
The presence of resveratrol (3,5,4'-trihydroxystilbene) and its beta-glucoside, piceid (resveratrol-3-beta-D-glucopyranoside), together with their isomers in wine appears to be one of the beneficial factors conferring a protective effect against cardiovascular disease through red wine ingestion. A total of 42 red and white wines was collected in areas from Hokkaido to Kyushu in Japan. The wines were fractionated with a C18 Sep-pak cartridge, and the active principles were eluted with ethyl acetate. Crude trans- and cis-piceid were extracted from a Chinese medicine, 'Kojohkon' (Polygonum cuspidatum), and their retention times and UV absorption were confirmed by HPLC. trans- and cis-Resveratrol, and trans- and cis-piceid were analyzed in a short C18 HPLC column, and cis-resveratrol was quantified from the amount of cis-isomer converted from authentic trans-resveratrol that had been treated by UV irradiation. The content of piceid is shown as the resveratrol equivalent. The average content of total stilbene compounds was 4.37 mg/liter in red wines, while only 0.68 mg/liter in white wines. Red wines made from Pinot noir, Merlot, and Zweigeltrebe grapes all had a high resveratrol content.
葡萄酒中白藜芦醇（3,5,4'-三羟基茋）及其β-葡萄糖苷、云杉新苷（白藜芦醇-3-β-D-吡喃葡萄糖苷）以及它们的异构体的存在，似乎是通过饮用红酒对心血管疾病产生保护作用的有益因素之一。在日本从北海道到九州的地区共收集了42种红葡萄酒和白葡萄酒。这些葡萄酒用C18 Sep-pak柱进行分离，活性成分用乙酸乙酯洗脱。从中药“虎杖”（虎杖）中提取粗制反式和顺式云杉新苷，并通过高效液相色谱法确认其保留时间和紫外吸收。在短C18高效液相色谱柱中分析反式和顺式白藜芦醇以及反式和顺式云杉新苷，通过紫外照射处理后的标准反式白藜芦醇转化的顺式异构体的量对顺式白藜芦醇进行定量。云杉新苷的含量以白藜芦醇当量表示。红葡萄酒中总芪化合物的平均含量为4.37毫克/升，而白葡萄酒中仅为0.68毫克/升。由黑皮诺、梅洛和茨威格葡萄制成的红葡萄酒白藜芦醇含量都很高。
