Nour Violeta, Trandafir Ion, Muntean Camelia
University of Craiova, Faculty of Horticulture, Dolj, Romania.
J Chromatogr Sci. 2012 Nov-Dec;50(10):920-7. doi: 10.1093/chromsci/bms091. Epub 2012 Jun 11.
A reversed-phase high-performance liquid chromatography method with diode array detection was developed for the determination of trans- and cis-resveratrol in red wines. Separation was achieved after direct injection by the use of a BDS Hypersil C18 column (250 × 4.6 mm) with gradient elution (solvent A: acetic acid 2%, solvent B: acetonitrile). Detection of trans- and cis-resveratrol was performed at 306 and 286 nm, respectively. The retention times of trans- and cis-resveratrol were 22.2 and 26.1 min, respectively. Good linearity and precision were obtained for the two isomers. Detection limits of 0.004 mg/L for trans-resveratrol and 0.02 mg/L for cis-reveratrol were obtained. The developed method was applied to determine cis- and trans-resveratrol in 30 red wines produced in Oltenia (southwestern Romania). The wines came from different vineyards harvested in various vintages. The concentration of trans-resveratrol ranged from 0.287 to 7.188 mg/L, while the content of cis-resveratrol ranged from 0.718 to 6.587 mg/L. The highest amount of trans-resveratrol was found in Merlot from Vanju Mare, Mehedinti (7.188 mg/L), followed by Sirah from Corcova, Mehedinti (4.738 mg/L), both from the 2010 harvest. The paper also approaches the study of the transformation of trans-resveratrol into the cis form after ultraviolet irradiation through glass and quartz. At the irradiation of a trans-resveratrol solution through quartz, the formation of another two compounds apart from cis-resveratrol was observed.
建立了一种采用二极管阵列检测的反相高效液相色谱法，用于测定红葡萄酒中的反式白藜芦醇和顺式白藜芦醇。直接进样后，使用BDS Hypersil C18柱（250×4.6 mm），通过梯度洗脱（溶剂A：2%乙酸，溶剂B：乙腈）实现分离。反式和顺式白藜芦醇分别在306和286 nm处进行检测。反式和顺式白藜芦醇的保留时间分别为22.2和26.1分钟。两种异构体均具有良好的线性和精密度。反式白藜芦醇的检测限为0.004 mg/L，顺式白藜芦醇的检测限为0.02 mg/L。所建立的方法用于测定罗马尼亚西南部奥尔特尼亚地区生产的30种红葡萄酒中的顺式和反式白藜芦醇。这些葡萄酒来自不同年份收获的不同葡萄园。反式白藜芦醇的浓度范围为0.287至7.188 mg/L，而顺式白藜芦醇的含量范围为0.718至6.587 mg/L。反式白藜芦醇含量最高的是来自梅赫丁蒂县万朱马雷的梅洛葡萄酒（7.188 mg/L），其次是来自梅赫丁蒂县科尔科瓦的西拉葡萄酒（4.738 mg/L），均为2010年收获。本文还探讨了反式白藜芦醇在通过玻璃和石英进行紫外线照射后转化为顺式的研究。通过石英照射反式白藜芦醇溶液时，除了顺式白藜芦醇外，还观察到另外两种化合物的形成。
