Nakai T, Tonouchi N, Tsuchida T, Mori H, Sakai F, Hayashi T
Wood Research Institute, Kyoto University, Japan.
Biosci Biotechnol Biochem. 1997 Nov;61(11):1955-6. doi: 10.1271/bbb.61.1955.
About 80% of radioactivity was recovered in asymmetrically labeled sucrose from UDP-[14C]glucose or [14C]fructose with recombinant mung bean sucrose synthase expressed in Escherichia coli harboring pEB-01. This high recovery is due to the fact that the enzyme conserving the activity of sucrose synthase has a similar affinity for UDP-glucose and fructose to an intact enzyme from the mung bean, but a lower affinity for sucrose.
用携带pEB - 01的大肠杆菌中表达的重组绿豆蔗糖合酶，从UDP - [14C]葡萄糖或[14C]果糖中获得的不对称标记蔗糖中回收了约80%的放射性。这种高回收率是由于以下事实：保留蔗糖合酶活性的该酶对UDP - 葡萄糖和果糖的亲和力与绿豆中的完整酶相似，但对蔗糖的亲和力较低。
