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1
Nucleotide G-1207 of 18S rRNA is an essential component of the human 80S ribosomal decoding center.18S核糖体RNA的核苷酸G-1207是人类80S核糖体解码中心的重要组成部分。
RNA. 1997 Dec;3(12):1480-5.
2
Nucleotides of 18S rRNA surrounding mRNA codons at the human ribosomal A, P, and E sites: a crosslinking study with mRNA analogs carrying an aryl azide group at either the uracil or the guanine residue.人核糖体A、P和E位点处围绕mRNA密码子的18S rRNA核苷酸：一项对在尿嘧啶或鸟嘌呤残基处带有芳基叠氮基团的mRNA类似物进行的交联研究。
RNA. 2000 Dec;6(12):1727-36. doi: 10.1017/s1355838200000996.
3
[Crosslinking of mRNA analog, pUUAGUAUUUAUU derivative with a photoactivated group at the guanosine residue, with human 80S ribosomes].[带有鸟苷残基上光活化基团的mRNA类似物pUUAGUAUUUAUU衍生物与人类80S核糖体的交联]
Mol Biol (Mosk). 2002 Jan-Feb;36(1):114-22.
4
[The mRNA codon environment at the P and E sites of human ribosomes deduced from photo crosslinking with pUUUGUU].[通过与pUUUGUU进行光交联推导得出的人核糖体P位和E位的mRNA密码子环境]
Mol Biol (Mosk). 2003 Jan-Feb;37(1):147-55.
5
[Positioning of mRNA 3' of the a site bound codon on the human 80S ribosome].[人80S核糖体上与A位点结合密码子3'端的mRNA定位]
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6
[C-domain of translation termination factor eRF1 neighbors stop codon at the 80S ribosomal A site].[翻译终止因子eRF1的C结构域在80S核糖体A位点与终止密码子相邻]
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[Affinity modification of 80S ribosomes from human placenta with mRNA analogs--derivatives of oligouridylates with and alkylating group at the 5'-end].
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8
The first position of a codon placed in the A site of the human 80S ribosome contacts nucleotide C1696 of the 18S rRNA as well as proteins S2, S3, S3a, S30, and S15.置于人类80S核糖体A位点的密码子的第一个位置与18S rRNA的核苷酸C1696以及蛋白质S2、S3、S3a、S30和S15接触。
Biochemistry. 2005 Feb 15;44(6):2153-62. doi: 10.1021/bi0487802.
9
[The first nucleotide of the codon located in the P site of the 80S ribosome is close to G1702 of the 18S rRNA as revealed by crosslinking to a pUUUGUU derivative containing a perfluorophenylazido group at the guanine].通过与在鸟嘌呤处含有全氟苯基叠氮基团的pUUUGUU衍生物交联显示，位于80S核糖体P位点的密码子的第一个核苷酸靠近18S rRNA的G1702。
Mol Biol (Mosk). 2004 May-Jun;38(3):501-6.
10
[Structural elements of 80S ribosomes located near the 5'-region of the mRNA-binding center].
Mol Biol (Mosk). 1994 Jul-Aug;28(4):918-25.

引用本文的文献

1
Structural rearrangements in mRNA upon its binding to human 80S ribosomes revealed by EPR spectroscopy.通过电子顺磁共振波谱学揭示了人 80S 核糖体结合的 mRNA 的结构重排。
Nucleic Acids Res. 2018 Jan 25;46(2):897-904. doi: 10.1093/nar/gkx1136.
2
General approach for introduction of various chemical labels in specific RNA locations based on insertion of amino linkers.基于插入氨基接头的方法，将各种化学标签引入到特定 RNA 位置的一般方法。
Molecules. 2013 Nov 25;18(12):14455-69. doi: 10.3390/molecules181214455.
3
Variable and conserved elements of human ribosomes surrounding the mRNA at the decoding and upstream sites.在解码位点和上游位点围绕mRNA的人类核糖体的可变和保守元件。
Nucleic Acids Res. 2004 Jun 18;32(11):3282-93. doi: 10.1093/nar/gkh657. Print 2004.
4
Nucleotides of 18S rRNA surrounding mRNA codons at the human ribosomal A, P, and E sites: a crosslinking study with mRNA analogs carrying an aryl azide group at either the uracil or the guanine residue.人核糖体A、P和E位点处围绕mRNA密码子的18S rRNA核苷酸：一项对在尿嘧啶或鸟嘌呤残基处带有芳基叠氮基团的mRNA类似物进行的交联研究。
RNA. 2000 Dec;6(12):1727-36. doi: 10.1017/s1355838200000996.
5
Proteins neighboring 18S rRNA conserved sequences 609-618 and 1047-1061 within the 40S human ribosomal subunit.在人40S核糖体亚基内与18S rRNA保守序列609 - 618和1047 - 1061相邻的蛋白质。
RNA. 1999 Dec;5(12):1656-64. doi: 10.1017/s1355838299990908.

18S核糖体RNA的核苷酸G-1207是人类80S核糖体解码中心的重要组成部分。

Nucleotide G-1207 of 18S rRNA is an essential component of the human 80S ribosomal decoding center.

作者信息

Bulygin K N, Graifer D M, Repkova M N, Smolenskaya I A, Veniyaminova A G, Karpova G G

机构信息

Novosibirsk Institute of Bioorganic Chemistry, Siberian Branch of Russian Academy of Sciences.

出版信息

RNA. 1997 Dec;3(12):1480-5.

PMID:9404898
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1369588/
Abstract

mRNA analogues-derivatives of oligoribonucleotides consisting of two different codons and bearing an aryl azide group at the 5'-phosphates-were crosslinked to human 80S ribosomes by UV-irradiation of the various model complexes obtained in the presence of the cognate tRNAs. Three sequences, namely pUUUGUU (coding for Phe and Val), pUUCUAAA (first triplet coding for Phe and second being stop-codon), and pGUGUUU (coding for Val and Phe), have been used. Sequences of 18S rRNA containing nucleotides crosslinked to the mRNA analogues were examined by hydrolysis with RNase H in the presence of various cDNA probes. Crosslinked nucleotides were identified by primer extension. In all cases, only nucleotide G-1207 (equivalent to G-926 in Escherichia coli 16S rRNA) has been detected as crosslinked. Crosslinking of the mRNA analogues to the large ribosomal subunit was negligible.

摘要

信使核糖核酸类似物——由两个不同密码子组成且在5'-磷酸处带有芳基叠氮基团的寡核糖核苷酸衍生物，通过对在同源转运核糖核酸存在下获得的各种模型复合物进行紫外线照射，与人类80S核糖体交联。使用了三个序列，即pUUUGUU（编码苯丙氨酸和缬氨酸）、pUUCUAAA（第一个三联体编码苯丙氨酸，第二个是终止密码子）和pGUGUUU（编码缬氨酸和苯丙氨酸）。在各种互补脱氧核糖核酸探针存在的情况下，通过核糖核酸酶H水解来检查与信使核糖核酸类似物交联的18S核糖体核糖核酸的序列。通过引物延伸鉴定交联的核苷酸。在所有情况下，仅检测到核苷酸G-1207（相当于大肠杆菌16S核糖体核糖核酸中的G-926）被交联。信使核糖核酸类似物与大核糖体亚基的交联可以忽略不计。