Rennekampff H O, Kiessig V, Griffey S, Greenleaf G, Hansbrough J F
Department of Surgery, the University of California, San Diego Medical Center, 92103-8896, USA.
J Burn Care Rehabil. 1997 Nov-Dec;18(6):535-44. doi: 10.1097/00004630-199711000-00012.
In full-thickness skin injury, loss of dermis may result in compromised wound repair, including contracture, hypertrophic scarring, and wound breakdown. This report examines the effect of an acellular dermal matrix on in vivo skin repair. Human keratinocytes cultured onto a synthetic hydrophilic dressing were applied with (N = 9) and without (N = 11) an acellular dermal matrix to full-thickness skin defects on athymic mice. Host cells progressively repopulated the acellular dermal component of the grafts. All animals with dermal matrix revealed fully differentiated epidermis by postoperative day 21. Human keratinocytes persisted in all animals grafted with dermal matrix, compared to only 63.6% of those animals without a dermal component. Planimetric analysis revealed significantly reduced wound contraction (p = 0.016) in animals receiving the dermal matrix. Histologic, immunohistochemical, and electron microscopic analyses also were performed. These studies suggest that an acellular dermal matrix can effectively direct regeneration of normal skin morphology.
在全层皮肤损伤中，真皮层的缺失可能会导致伤口修复受损，包括挛缩、肥厚性瘢痕形成和伤口裂开。本报告研究了脱细胞真皮基质对体内皮肤修复的影响。将培养在合成亲水性敷料上的人角质形成细胞应用于无胸腺小鼠的全层皮肤缺损，一组使用脱细胞真皮基质（N = 9），另一组不使用（N = 11）。宿主细胞逐渐重新填充移植物的脱细胞真皮成分。到术后第21天，所有使用真皮基质的动物均显示出完全分化的表皮。与未使用真皮成分的动物中只有63.6%相比，在所有移植了真皮基质的动物中，人角质形成细胞均持续存在。平面测量分析显示，接受真皮基质的动物伤口收缩明显减少（p = 0.016）。还进行了组织学、免疫组织化学和电子显微镜分析。这些研究表明，脱细胞真皮基质可以有效地引导正常皮肤形态的再生。
