Kuriyama H, Kawamoto S, Ishida N, Ohno I, Mita S, Matsuzawa Y, Matsubara K, Okubo K
Institute for Molecular and Cellular Biology, Osaka University, Osaka, Suita, 565, Japan.
Biochem Biophys Res Commun. 1997 Dec 8;241(1):53-8. doi: 10.1006/bbrc.1997.7769.
In a systematic analysis of genes expressed in human adipose tissue, we detected a novel gene that is expressed uniquely in adipose tissue. The sequence showed that it encodes a 342-amino-acid protein containing six putative transmembrane domains, and is a new member of the aquaporin family of water-selective membrane channels. We named this gene aquaporin 9. It features a cyclic-AMP protein kinase phosphorylation consensus site in the NH3-terminal domain. Expression of the cRNA in Xenopus oocytes yielded a 7-fold increase in osmotic water permeability blocked by 0.3 mM HgCl2, and also facilitated the uptake of glycerol. Northern blot analysis demonstrated that the mRNA is abundant in adipose tissue, but not in other tissues. Thus, this gene product may participate in glycerol transport in adipocytes.
在一项对人类脂肪组织中表达基因的系统分析中，我们检测到一个在脂肪组织中独特表达的新基因。序列分析表明，它编码一个含有六个假定跨膜结构域的342个氨基酸的蛋白质，是水选择性膜通道水通道蛋白家族的一个新成员。我们将该基因命名为水通道蛋白9。它在NH3末端结构域具有一个环磷酸腺苷蛋白激酶磷酸化共有位点。在非洲爪蟾卵母细胞中表达cRNA可使渗透水通透性增加7倍，该增加可被0.3 mM HgCl2阻断，并且还促进了甘油的摄取。Northern印迹分析表明，该mRNA在脂肪组织中丰富，但在其他组织中不丰富。因此，该基因产物可能参与脂肪细胞中的甘油转运。
