嗜酸性粒细胞趋化因子对内皮细胞表面黏附分子及嗜酸性粒细胞与微血管内皮细胞黏附的影响。
The effects of eotaxin on the surface adhesion molecules of endothelial cells and on eosinophil adhesion to microvascular endothelial cells.
作者信息
Hohki G, Terada N, Hamano N, Kitaura M, Nakajima T, Yoshie O, Ikeda T, Kimura S, Konno A
机构信息
Department of Otorhinolaryngology, Chiba University School of Medicine, Chiba, 260, Japan.
出版信息
Biochem Biophys Res Commun. 1997 Dec 8;241(1):136-41. doi: 10.1006/bbrc.1997.7785.
Eosinophil recruitment occurs in tissues as the result of allergic diseases. Human eotaxin is thought to be specific to eosinophils. In this study, we examined the effects of human eotaxin on the expression of adhesion molecules on nasal microvascular endothelial cells and on eosinophil adhesion to endothelial cells. Eotaxin upregulated the expression of ICAM-1 and VCAM-1 on human nasal mucosal microvascular endothelial cells (HMMEC), but not human umbilical vein endothelial cells (HUVEC). The eotaxin-induced eosinophil adhesion to HMMEC was increased at 10 ng/ml and significantly increased at the concentration of 100 ng/ml. On HUVEC, however, eotaxin did not induce increases of eosinophil adhesion. Anti-ICAM-1 and anti-VCAM-1 mAbs significantly decreased eotaxin-induced eosinophil adhesion. These results suggest that eotaxin regulates eosinophil accumulation to the nasal mucosa through its effect on the adhesion molecules on microvascular endothelial cells.
嗜酸性粒细胞的募集是过敏性疾病导致的结果，发生在组织中。人嗜酸性粒细胞趋化因子被认为对嗜酸性粒细胞具有特异性。在本研究中，我们检测了人嗜酸性粒细胞趋化因子对鼻微血管内皮细胞黏附分子表达以及对嗜酸性粒细胞与内皮细胞黏附的影响。嗜酸性粒细胞趋化因子上调了人鼻黏膜微血管内皮细胞（HMMEC）上细胞间黏附分子-1（ICAM-1）和血管细胞黏附分子-1（VCAM-1）的表达，但对人脐静脉内皮细胞（HUVEC）没有影响。嗜酸性粒细胞趋化因子诱导嗜酸性粒细胞与HMMEC的黏附在10 ng/ml时增加，在100 ng/ml浓度时显著增加。然而，在HUVEC上，嗜酸性粒细胞趋化因子并未诱导嗜酸性粒细胞黏附增加。抗ICAM-1和抗VCAM-1单克隆抗体显著降低了嗜酸性粒细胞趋化因子诱导的嗜酸性粒细胞黏附。这些结果表明，嗜酸性粒细胞趋化因子通过对微血管内皮细胞上黏附分子的作用来调节嗜酸性粒细胞在鼻黏膜的积聚。
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