拟南芥NPH1：一种具有假定氧化还原感应结构域的蛋白激酶。

Arabidopsis NPH1: a protein kinase with a putative redox-sensing domain.

作者信息

Huala E, Oeller P W, Liscum E, Han I S, Larsen E, Briggs W R

机构信息

Department of Plant Biology, Carnegie Institution of Washington, 260 Panama Street, Stanford, CA 94305, USA.

出版信息

Science. 1997 Dec 19;278(5346):2120-3. doi: 10.1126/science.278.5346.2120.

DOI:10.1126/science.278.5346.2120

PMID:9405347

Abstract

The NPH1 (nonphototropic hypocotyl 1) gene encodes an essential component acting very early in the signal-transduction chain for phototropism. Arabidopsis NPH1 contains a serine-threonine kinase domain and LOV1 and LOV2 repeats that share similarity (36 to 56 percent) with Halobacterium salinarium Bat, Azotobacter vinelandii NIFL, Neurospora crassa White Collar-1, Escherichia coli Aer, and the Eag family of potassium-channel proteins from Drosophila and mammals. Sequence similarity with a known (NIFL) and a suspected (Aer) flavoprotein suggests that NPH1 LOV1 and LOV2 may be flavin-binding domains that regulate kinase activity in response to blue light-induced redox changes.

摘要

NPH1（非向光性下胚轴1）基因编码一种在向光性信号转导链中起作用非常早的必需成分。拟南芥NPH1包含一个丝氨酸 - 苏氨酸激酶结构域以及与盐生盐杆菌Bat、棕色固氮菌NIFL、粗糙脉孢菌白领-1、大肠杆菌Aer以及果蝇和哺乳动物的钾通道蛋白Eag家族具有相似性（36%至56%）的LOV1和LOV2重复序列。与已知的（NIFL）和疑似的（Aer）黄素蛋白的序列相似性表明，NPH1的LOV1和LOV2可能是黄素结合结构域，可响应蓝光诱导的氧化还原变化来调节激酶活性。