在脊椎动物系统中由Reaper诱导的细胞凋亡。
Reaper-induced apoptosis in a vertebrate system.
作者信息
Evans E K, Kuwana T, Strum S L, Smith J J, Newmeyer D D, Kornbluth S
机构信息
Department of Pharmacology and Cancer Biology, Duke University Medical Center, Box 3686, C366 LSRC, Research Drive, Durham, NC 27710, USA.
出版信息
EMBO J. 1997 Dec 15;16(24):7372-81. doi: 10.1093/emboj/16.24.7372.
Abstract
The reaper protein of Drosophila melanogaster has been shown to be a central regulator of apoptosis in that organism. However, it has not been shown to function in any vertebrate nor have the cellular components required for its action been defined. In this report we show that reaper can induce rapid apoptosis in vitro using an apoptotic reconstitution system derived from Xenopus eggs. Moreover, we show that a subcellular fraction enriched in mitochondria is required for this process and that reaper, acting in conjunction with cytosolic factors, can trigger mitochondrial cytochrome c release. Bcl-2 antagonizes these effects, but high levels of reaper can overcome the Bcl-2 block. These results demonstrate that reaper can function in a vertebrate context, suggesting that reaper-responsive factors are conserved elements of the apoptotic program.
摘要
黑腹果蝇的收割者蛋白已被证明是该生物体中细胞凋亡的核心调节因子。然而，它尚未在任何脊椎动物中发挥作用，其作用所需的细胞成分也未明确。在本报告中，我们表明收割者可以利用源自非洲爪蟾卵的凋亡重建系统在体外诱导快速细胞凋亡。此外，我们表明该过程需要富含线粒体的亚细胞组分，并且收割者与胞质因子协同作用可触发线粒体细胞色素c的释放。Bcl-2可拮抗这些作用，但高水平的收割者可克服Bcl-2的阻断作用。这些结果表明收割者可在脊椎动物环境中发挥作用，这表明对收割者有反应的因子是凋亡程序中的保守元件。
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本文引用的文献
1
The Bcl-2 family of proteins: regulators of cell death and survival.Bcl-2蛋白家族：细胞死亡与存活的调节因子。
Trends Cell Biol. 1994 Nov;4(11):399-403. doi: 10.1016/0962-8924(94)90053-1.
2
Cytochrome c activation of CPP32-like proteolysis plays a critical role in a Xenopus cell-free apoptosis system.细胞色素c对CPP32样蛋白水解的激活在非洲爪蟾无细胞凋亡系统中起关键作用。
EMBO J. 1997 Aug 1;16(15):4639-49. doi: 10.1093/emboj/16.15.4639.
3
Temporal phases in apoptosis defined by the actions of Src homology 2 domains, ceramide, Bcl-2, interleukin-1beta converting enzyme family proteases, and a dense membrane fraction.由Src同源2结构域、神经酰胺、Bcl-2、白细胞介素-1β转换酶家族蛋白酶和致密膜组分的作用所定义的细胞凋亡中的时间阶段。
J Cell Biol. 1997 Jun 2;137(5):1117-25. doi: 10.1083/jcb.137.5.1117.
4
DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis.DFF是一种异二聚体蛋白，在细胞凋亡过程中，它在半胱天冬酶-3下游发挥作用，触发DNA片段化。
Cell. 1997 Apr 18;89(2):175-84. doi: 10.1016/s0092-8674(00)80197-x.
5
Crk is required for apoptosis in Xenopus egg extracts.非洲爪蟾卵提取物中的细胞凋亡需要Crk。
EMBO J. 1997 Jan 15;16(2):230-41. doi: 10.1093/emboj/16.2.230.
6
The release of cytochrome c from mitochondria: a primary site for Bcl-2 regulation of apoptosis.细胞色素c从线粒体的释放：Bcl-2调控细胞凋亡的主要位点。
Science. 1997 Feb 21;275(5303):1132-6. doi: 10.1126/science.275.5303.1132.
7
Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blocked.Bcl-2对细胞凋亡的预防作用：线粒体中细胞色素c的释放受阻。
Science. 1997 Feb 21;275(5303):1129-32. doi: 10.1126/science.275.5303.1129.
8
Interaction of CED-4 with CED-3 and CED-9: a molecular framework for cell death.CED-4与CED-3和CED-9的相互作用：细胞死亡的分子框架。
Science. 1997 Feb 21;275(5303):1122-6. doi: 10.1126/science.275.5303.1122.
9
Interaction between the C. elegans cell-death regulators CED-9 and CED-4.秀丽隐杆线虫细胞死亡调节因子CED-9与CED-4之间的相互作用。
Nature. 1997 Feb 13;385(6617):653-6. doi: 10.1038/385653a0.
10
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Mol Cell Biol. 1997 Feb;17(2):667-76. doi: 10.1128/MCB.17.2.667.
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