Chanama S, Crawford R L
University of Idaho Institute for Molecular and Agricultural Genetic Engineering, Moscow 83844-1052, USA.
Appl Environ Microbiol. 1997 Dec;63(12):4833-8. doi: 10.1128/aem.63.12.4833-4838.1997.
Sphingomonas (Flavobacterium) chlorophenolica ATCC 39723 degrades pentachlorophenol (PCP) through a catabolic pathway encoded by multiple genes. One gene required for PCP degradation is pcpA, which encodes information for a 30-kDa polypeptide, PcpA, found in the periplasm of the bacterium. The biological role of PcpA has remained unknown. We disrupted pcpA by replacing it with a defective copy through homologous recombination. The pcpA recombinant, mutant strains accumulated 2,6-dichlorohydroquinone (2,6-DiCH) as a metabolite of PCP. This work confirms that pcpA is essential for degradation of PCP by S. chlorophenolica ATCC 39723 and suggests that it encodes a protein involved in hydrolytic dehalogenation of 2,6-DiCH, an already established primary metabolite of the PCP catabolic pathway.
嗜麦芽寡养单胞菌（黄杆菌属）嗜氯酚亚种ATCC 39723通过多个基因编码的分解代谢途径降解五氯酚（PCP）。PCP降解所需的一个基因是pcpA，它编码一种30 kDa多肽PcpA的信息，该多肽存在于细菌的周质中。PcpA的生物学作用尚不清楚。我们通过同源重组用一个缺陷拷贝替换pcpA，从而破坏了pcpA。pcpA重组突变菌株积累了2,6-二氯对苯二酚（2,6-DiCH）作为PCP的代谢产物。这项工作证实了pcpA对嗜麦芽寡养单胞菌ATCC 39723降解PCP至关重要，并表明它编码一种参与2,6-DiCH水解脱卤的蛋白质，2,6-DiCH是PCP分解代谢途径中已确定的主要代谢产物。
