嗜氯鞘氨醇单胞菌（黄杆菌属）ATCC 39723中pcpA的突变分析及其在五氯苯酚降解中的作用
Mutational analysis of pcpA and its role in pentachlorophenol degradation by Sphingomonas (Flavobacterium) chlorophenolica ATCC 39723.
作者信息
Chanama S, Crawford R L
机构信息
University of Idaho Institute for Molecular and Agricultural Genetic Engineering, Moscow 83844-1052, USA.
出版信息
Appl Environ Microbiol. 1997 Dec;63(12):4833-8. doi: 10.1128/aem.63.12.4833-4838.1997.
Abstract
Sphingomonas (Flavobacterium) chlorophenolica ATCC 39723 degrades pentachlorophenol (PCP) through a catabolic pathway encoded by multiple genes. One gene required for PCP degradation is pcpA, which encodes information for a 30-kDa polypeptide, PcpA, found in the periplasm of the bacterium. The biological role of PcpA has remained unknown. We disrupted pcpA by replacing it with a defective copy through homologous recombination. The pcpA recombinant, mutant strains accumulated 2,6-dichlorohydroquinone (2,6-DiCH) as a metabolite of PCP. This work confirms that pcpA is essential for degradation of PCP by S. chlorophenolica ATCC 39723 and suggests that it encodes a protein involved in hydrolytic dehalogenation of 2,6-DiCH, an already established primary metabolite of the PCP catabolic pathway.
摘要
嗜麦芽寡养单胞菌（黄杆菌属）嗜氯酚亚种ATCC 39723通过多个基因编码的分解代谢途径降解五氯酚（PCP）。PCP降解所需的一个基因是pcpA，它编码一种30 kDa多肽PcpA的信息，该多肽存在于细菌的周质中。PcpA的生物学作用尚不清楚。我们通过同源重组用一个缺陷拷贝替换pcpA，从而破坏了pcpA。pcpA重组突变菌株积累了2,6-二氯对苯二酚（2,6-DiCH）作为PCP的代谢产物。这项工作证实了pcpA对嗜麦芽寡养单胞菌ATCC 39723降解PCP至关重要，并表明它编码一种参与2,6-DiCH水解脱卤的蛋白质，2,6-DiCH是PCP分解代谢途径中已确定的主要代谢产物。
相似文献
1
Mutational analysis of pcpA and its role in pentachlorophenol degradation by Sphingomonas (Flavobacterium) chlorophenolica ATCC 39723.嗜氯鞘氨醇单胞菌（黄杆菌属）ATCC 39723中pcpA的突变分析及其在五氯苯酚降解中的作用
Appl Environ Microbiol. 1997 Dec;63(12):4833-8. doi: 10.1128/aem.63.12.4833-4838.1997.
2
Evidence that pcpA encodes 2,6-dichlorohydroquinone dioxygenase, the ring cleavage enzyme required for pentachlorophenol degradation in Sphingomonas chlorophenolica strain ATCC 39723.有证据表明，pcpA编码2,6 - 二氯对苯二酚双加氧酶，这是嗜氯鞘氨醇菌（Sphingomonas chlorophenolica）菌株ATCC 39723中五氯苯酚降解所需的环裂解酶。
Biochemistry. 1999 Jun 15;38(24):7659-69. doi: 10.1021/bi990103y.
3
PcpA, which is involved in the degradation of pentachlorophenol in Sphingomonas chlorophenolica ATCC39723, is a novel type of ring-cleavage dioxygenase.PcpA参与了嗜氯鞘氨醇单胞菌ATCC39723中五氯苯酚的降解，是一种新型的环裂解双加氧酶。
FEBS Lett. 1999 Oct 15;459(3):395-8. doi: 10.1016/s0014-5793(99)01305-8.
4
Characterization of 2,6-dichloro-p-hydroquinone 1,2-dioxygenase (PcpA) of Sphingomonas chlorophenolica ATCC 39723.嗜氯鞘氨醇单胞菌ATCC 39723的2,6-二氯对苯二酚1,2-双加氧酶（PcpA）的特性分析
Biochem Biophys Res Commun. 1999 Dec 20;266(2):322-5. doi: 10.1006/bbrc.1999.1805.
5
Organization and regulation of pentachlorophenol-degrading genes in Sphingobium chlorophenolicum ATCC 39723.嗜氯鞘氨醇菌ATCC 39723中五氯苯酚降解基因的组织与调控
J Bacteriol. 2002 Sep;184(17):4672-80. doi: 10.1128/JB.184.17.4672-4680.2002.
6
Isolation and physiological characterization of the pentachlorophenol degrading bacterium Sphingomonas chlorophenolica.五氯苯酚降解菌嗜氯鞘氨醇单胞菌的分离及生理特性研究
Chemosphere. 2006 Feb;62(5):709-14. doi: 10.1016/j.chemosphere.2005.05.012. Epub 2005 Jul 11.
7
Molecular analysis of pentachlorophenol degradation.五氯苯酚降解的分子分析
Biodegradation. 1994 Dec;5(3-4):277-88. doi: 10.1007/BF00696465.
8
Purification of a Flavobacterium pentachlorophenol-induced periplasmic protein (PcpA) and nucleotide sequence of the corresponding gene (pcpA).
J Bacteriol. 1991 May;173(9):2920-6. doi: 10.1128/jb.173.9.2920-2926.1991.
9
Dual substrate biodegradation of a nonionic surfactant and pentachlorophenol by Sphingomonas chlorophenolica RA2.嗜氯鞘氨醇单胞菌RA2对非离子表面活性剂和五氯苯酚的双底物生物降解作用
Biotechnol Bioeng. 2005 Mar 20;89(6):680-9. doi: 10.1002/bit.20365.
10
Maintenance role of a glutathionyl-hydroquinone lyase (PcpF) in pentachlorophenol degradation by Sphingobium chlorophenolicum ATCC 39723.谷胱甘肽基对苯二酚裂解酶（PcpF）在嗜氯酚鞘氨醇菌ATCC 39723降解五氯酚中的维持作用
J Bacteriol. 2008 Dec;190(23):7595-600. doi: 10.1128/JB.00489-08. Epub 2008 Sep 26.
引用本文的文献
1
Study on the biodegradation of persistent organic pollutants (POPs).持久性有机污染物（POPs）的生物降解研究。
J Pestic Sci. 2020 May 20;45(2):119-123. doi: 10.1584/jpestics.J19-06.
2
Sphingobium chlorophenolicum dichlorohydroquinone dioxygenase (PcpA) is alkaline resistant and thermally stable.地杆菌属邻氯对苯二酚双加氧酶（PcpA）具有耐碱性和热稳定性。
Int J Biol Sci. 2011;7(8):1171-9. doi: 10.7150/ijbs.7.1171. Epub 2011 Oct 25.
3
Identification of quinoide redox mediators that are formed during the degradation of naphthalene-2-sulfonate by Sphingomonas xenophaga BN6.对食异源鞘氨醇单胞菌BN6降解萘-2-磺酸盐过程中形成的醌类氧化还原介质的鉴定。
Appl Environ Microbiol. 2002 Sep;68(9):4341-9. doi: 10.1128/AEM.68.9.4341-4349.2002.
4
Organization and regulation of pentachlorophenol-degrading genes in Sphingobium chlorophenolicum ATCC 39723.嗜氯鞘氨醇菌ATCC 39723中五氯苯酚降解基因的组织与调控
J Bacteriol. 2002 Sep;184(17):4672-80. doi: 10.1128/JB.184.17.4672-4680.2002.
5
Cloning and sequencing of a novel meta-cleavage dioxygenase gene whose product is involved in degradation of gamma-hexachlorocyclohexane in Sphingomonas paucimobilis.一种新型间位裂解双加氧酶基因的克隆与测序，该基因产物参与少动鞘氨醇单胞菌中γ-六氯环己烷的降解
J Bacteriol. 1999 Nov;181(21):6712-9. doi: 10.1128/JB.181.21.6712-6719.1999.
本文引用的文献
1
Purification and characterization of 2,6-dichloro-p-hydroquinone chlorohydrolase from Flavobacterium sp. strain ATCC 39723.来自黄杆菌属菌株ATCC 39723的2,6-二氯对苯二酚氯水解酶的纯化与特性分析
J Bacteriol. 1997 Mar;179(5):1521-4. doi: 10.1128/jb.179.5.1521-1524.1997.
2
Characterization of a Flavobacterium glutathione S-transferase gene involved reductive dechlorination.一种参与还原脱氯的黄杆菌谷胱甘肽S-转移酶基因的特性分析。
J Bacteriol. 1993 May;175(9):2640-4. doi: 10.1128/jb.175.9.2640-2644.1993.
3
Cloning, sequence analysis, and expression of the Flavobacterium pentachlorophenol-4-monooxygenase gene in Escherichia coli.嗜麦芽寡养单胞菌五氯苯酚-4-单加氧酶基因在大肠杆菌中的克隆、序列分析及表达
J Bacteriol. 1993 Jan;175(2):411-6. doi: 10.1128/jb.175.2.411-416.1993.
4
Isolation and characterization of Flavobacterium strains that degrade pentachlorophenol.降解五氯苯酚的黄杆菌菌株的分离与鉴定
Appl Environ Microbiol. 1985 Dec;50(6):1512-8. doi: 10.1128/aem.50.6.1512-1518.1985.
5
Catabolism of pentachlorophenol by a Flavobacterium sp.黄杆菌属细菌对五氯苯酚的分解代谢
Biochem Biophys Res Commun. 1986 Dec 15;141(2):825-30. doi: 10.1016/s0006-291x(86)80247-9.
6
Metabolism of gallate and phloroglucinol in Eubacterium oxidoreducens via 3-hydroxy-5-oxohexanoate.没食子酸盐和间苯三酚在氧化还原真杆菌中通过3-羟基-5-氧代己酸的代谢。
J Bacteriol. 1987 May;169(5):1886-90. doi: 10.1128/jb.169.5.1886-1890.1987.
7
High frequency targeting of genes to specific sites in the mammalian genome.将基因高频靶向哺乳动物基因组中的特定位点。
Cell. 1986 Feb 14;44(3):419-28. doi: 10.1016/0092-8674(86)90463-0.
8
Genomic replacement in Escherichia coli K-12 using covalently closed circular plasmid DNA.利用共价闭合环状质粒DNA在大肠杆菌K-12中进行基因组替换
Gene. 1990 Nov 30;96(1):29-36. doi: 10.1016/0378-1119(90)90337-q.
9
Extrachromosomal homologous recombination and gene targeting in plant cells after Agrobacterium mediated transformation.农杆菌介导转化后植物细胞中的染色体外同源重组和基因靶向
EMBO J. 1990 Oct;9(10):3077-84. doi: 10.1002/j.1460-2075.1990.tb07504.x.
10
Purification and properties of pentachlorophenol hydroxylase, a flavoprotein from Flavobacterium sp. strain ATCC 39723.来自黄杆菌属菌株ATCC 39723的黄素蛋白——五氯苯酚羟化酶的纯化及性质
J Bacteriol. 1991 Jul;173(14):4447-53. doi: 10.1128/jb.173.14.4447-4453.1991.
Zotero 插件