Orser C S, Lange C C
Xenometrix, Inc., Boulder, CO 80301.
Biodegradation. 1994 Dec;5(3-4):277-88. doi: 10.1007/BF00696465.
A limited number of microorganisms have been described for their ability to partially degrade pentachlorophenol (PCP), or to completely mineralize it. Several years ago we chose one of these microorganisms, Flavobacterium sp. strain ATCC 39723, for use in a detailed molecular analysis of the catabolism of PCP. This strain was chosen because it had previously been studied in great detail for its growth characteristics in relation to degradation of PCP. In this paper we provide an overview of the degradation pathway of PCP to 2,6-dichloro-p-hydroquinone by Flavobacterium. The specific biochemical reactions and the genes encoding the enzymes are reviewed. The successful transformation and site specific mutagenesis of Flavobacterium, as well as the discovery of two new pcp alleles is also presented.
仅有少数微生物因其能够部分降解五氯苯酚（PCP）或使其完全矿化的能力而被描述。几年前，我们选择了其中一种微生物，即黄杆菌属菌株ATCC 39723，用于对PCP分解代谢进行详细的分子分析。选择该菌株是因为之前已经对其与PCP降解相关的生长特性进行了深入研究。在本文中，我们概述了黄杆菌将PCP降解为2,6-二氯对苯二酚的途径。对具体的生化反应和编码这些酶的基因进行了综述。还介绍了黄杆菌的成功转化和位点特异性诱变，以及两个新的pcp等位基因的发现。
