位于19号染色体上的人类锌指基因在精原细胞瘤中表达增强。

Enhanced expression in seminoma of human zinc finger genes located on chromosome 19.

作者信息

Ogawa T, Poncelet D A, Kinoshita Y, Noce T, Takeda M, Kawamoto K, Udagawa K, Lecocq P J, Marine J C, Martial J A, Hosaka M

机构信息

Department of Urology, Yokohama City University, School of Medicine, Japan.

出版信息

Cancer Genet Cytogenet. 1998 Jan 1;100(1):36-42. doi: 10.1016/s0165-4608(97)00004-6.

DOI:10.1016/s0165-4608(97)00004-6

PMID:9406578

Abstract

Six Krüppel-type zinc finger (ZF) genes were cloned from a seminoma cDNA library. One, ZFS-1, showed high sequence homology to the ZNF91 KRAB (Krüppel-associated box) ZF gene family and also the same chromosomal assignment. Interestingly, Northern blot analyses using ZFS-1 and ZNF91 revealed that multiple ZF genes on chromosome 19 were predominantly expressed in seminomas. In addition, the testis and the seminoma showed specific expression of 2.3 kb transcript. Our results suggest that ZF genes on chromosome 19 may be implicated in the development and/or growth of seminomas.

摘要

从精原细胞瘤cDNA文库中克隆出六个Krüppel型锌指（ZF）基因。其中一个基因ZFS-1，与ZNF91 KRAB（Krüppel相关盒）ZF基因家族具有高度的序列同源性，并且染色体定位相同。有趣的是，使用ZFS-1和ZNF91进行的Northern印迹分析表明，19号染色体上的多个ZF基因主要在精原细胞瘤中表达。此外，睾丸和精原细胞瘤显示出2.3 kb转录本的特异性表达。我们的结果表明，19号染色体上的ZF基因可能与精原细胞瘤的发生和/或生长有关。