通过间接荧光抗体试验和不同的酶联免疫吸附测定评估牛新孢子虫病暴发期间的抗体反应。
Antibody responses of cows during an outbreak of neosporosis evaluated by indirect fluorescent antibody test and different enzyme-linked immunosorbent assays.
作者信息
Dubey J P, Jenkins M C, Adams D S, McAllister M M, Anderson-Sprecher R, Baszler T V, Kwok O C, Lally N C, Björkman C, Uggla A
机构信息
United States Department of Agriculture, Agricultural Research Service, Livestock and Poultry Sciences Institute, Beltsville, Maryland 20705-2350, USA.
出版信息
J Parasitol. 1997 Dec;83(6):1063-9.
Serum samples from 70 (33 aborting and 37 non-aborting) dairy cows from a herd in California were analyzed for Neospora caninum antibodies in different laboratories by various serologic assays including enzyme-linked immunosorbent assay (ELISA) with recombinant antigens (Nc4.1 and Nc14.1), kinetic ELISA, whole tachyzoite lysate ELISA, immunostimulating complex (iscom) ELISA, antigen capture competitive inhibition ELISA, and by the indirect fluorescent antibody test. Eighteen percent of pregnant cows in this herd had aborted within 2 mo of the index case. All 70 cows had antibodies to N. caninum by at least 1 of the tests. Antibody levels to N. caninum in aborting cows as a group were higher than in nonaborting cows. However, it was concluded that no serological test could be used to establish definitively that N. caninum caused the abortion in an individual cow.
来自加利福尼亚一群奶牛中的70头（33头流产和37头未流产）奶牛的血清样本，在不同实验室通过各种血清学检测方法进行了新孢子虫抗体分析，这些方法包括使用重组抗原（Nc4.1和Nc14.1）的酶联免疫吸附测定（ELISA）、动力学ELISA、全速殖子裂解物ELISA、免疫刺激复合物（iscom）ELISA、抗原捕获竞争抑制ELISA，以及间接荧光抗体试验。该牛群中18%的怀孕母牛在索引病例后的2个月内流产。所有70头奶牛至少通过一种检测方法检测出对新孢子虫有抗体。流产奶牛群体中针对新孢子虫的抗体水平高于未流产奶牛。然而，得出的结论是，没有一种血清学检测方法能够明确确定新孢子虫导致了某一头奶牛的流产。
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