Volety A K, Chu F L
Virginia Institute of Marine Science, School of Marine Science, College of William & Mary, Gloucester Point 23062, USA.
J Parasitol. 1997 Dec;83(6):1093-8.
The effect of temperature (4, 12, 20, and 28 C) and osmolality (400, 570, and 840 mOsm/kg) on extracellular acid phosphatase (AP) secretion in vitro, and ultrastructural localization of AP activity in the parasite were investigated. The extracellular AP secretion by Perkinsus marinus was cell density dependent (P < 0.001). Increasing culture temperatures resulted in increased P. marinus proliferation concomitant with AP secretion (P < 0.0001). AP secretion was similar in P. marinus cultured at 400 and 570 mOsm/kg media, but higher than P. marinus cultured at 870 mOsm/kg media. Results of the ultrastructural study revealed that intense AP activity was in the nucleus of the parasite. Based on its distribution in the nucleus, AP may be playing a role in events leading to cell cycle regulation.
研究了温度（4、12、20和28℃）和渗透压（400、570和840 mOsm/kg）对体外细胞外酸性磷酸酶（AP）分泌的影响，以及该寄生虫中AP活性的超微结构定位。海派金藻的细胞外AP分泌呈细胞密度依赖性（P < 0.001）。培养温度升高导致海派金藻增殖增加，同时AP分泌增加（P < 0.0001）。在400和570 mOsm/kg培养基中培养的海派金藻的AP分泌相似，但高于在870 mOsm/kg培养基中培养的海派金藻。超微结构研究结果显示，该寄生虫的细胞核中有强烈的AP活性。基于其在细胞核中的分布，AP可能在导致细胞周期调控的事件中发挥作用。
