Hisano S, Haga H, Li Z, Tatsumi S, Miyamoto K I, Takeda E, Fukui Y
Department of Anatomy, School of Medicine, University of Tokushima, Japan.
Brain Res. 1997 Oct 24;772(1-2):149-55. doi: 10.1016/s0006-8993(97)00884-6.
Expression of a renal Na+-dependent inorganic phosphate (Pi) cotransporter (NaPi-2) was studied in rat forebrain with reverse transcription and polymerase chain reaction (RT-PCR) and immunohistochemistry. RT-PCR analysis for total RNA from whole brain and sequencing of the PCR products showed expression of NaPi-2 mRNA in the brain. Immunohistochemical analysis revealed NaPi-2 staining in many nonpyramidal neurons of all six layers throughout neocortical areas and in neurons of proisocortical and periallocortical areas. NaPi-2-immunoreactive neurons were also detectable in the piriform cortex, hippocampal formation, caudate-putamen, amygdaloid nuclei and lateral geniculate nucleus. Furthermore, NaPi-2 staining was shown in ependymal cells and microvascular endothelial cells. The present results suggest that NaPi-2 is synthesized within the brain and involved in maintaining Pi homeostasis of certain neurons and/or the entire brain.
采用逆转录聚合酶链反应（RT-PCR）和免疫组织化学方法，研究大鼠前脑中肾钠依赖性无机磷酸盐（Pi）共转运体（NaPi-2）的表达。对全脑总RNA进行RT-PCR分析及PCR产物测序，结果显示脑中存在NaPi-2 mRNA表达。免疫组织化学分析表明，在新皮质区域所有六层的许多非锥体神经元以及原皮质和周围allocortical区域的神经元中均有NaPi-2染色。在梨状皮质、海马结构、尾状核-壳核、杏仁核和外侧膝状体核中也可检测到NaPi-2免疫反应性神经元。此外，室管膜细胞和微血管内皮细胞中也显示有NaPi-2染色。目前的结果表明，NaPi-2在脑内合成，并参与维持某些神经元和/或整个脑的Pi稳态。
