Singhal P C, Franki N, Kumari S, Sanwal V, Wagner J D, Mattana J
Department of Medicine, Long Island Jewish Medical Center, New Hyde Park, New York 11040, USA.
J Cell Biochem. 1998 Jan 1;68(1):22-30. doi: 10.1002/(sici)1097-4644(19980101)68:1<22::aid-jcb3>3.0.co;2-y.
Mesangial matrix is a dynamic structure which modulates mesangial cell function. Since accumulation of matrix precedes the development of focal glomerulosclerosis, we studied the effect of different matrices on mesangial cell (MC) apoptosis. Suspended mesangial cells became apoptotic in a time dependent manner. Collagen type III did not modulate MC apoptosis when compared to cells grown on plastic. MCs grown on Matrigel, collagen type I and IV showed an increased number of apoptotic cells when compared to MCs grown on plastic. DNA end-labeling further confirmed these observations. MCs grown on Matrigel showed enhanced (P < 0.05) mRNA expression for tissue transglutaminase (TTG) and cathepsin-B. Mesangial cells grown on Matrigel also showed enhanced expression of superoxide dismutase (SOD). We conclude that mesangial cells require attachment to the matrix for their survival and alteration of the quality of matrix modulates mesangial cell apoptosis.
系膜基质是一种动态结构，可调节系膜细胞功能。由于基质的积累先于局灶性肾小球硬化的发展，我们研究了不同基质对系膜细胞（MC）凋亡的影响。悬浮的系膜细胞以时间依赖性方式发生凋亡。与在塑料上生长的细胞相比，III型胶原不调节MC凋亡。与在塑料上生长的MC相比，在基质胶、I型和IV型胶原上生长的MC显示凋亡细胞数量增加。DNA末端标记进一步证实了这些观察结果。在基质胶上生长的MC显示组织转谷氨酰胺酶（TTG）和组织蛋白酶B的mRNA表达增强（P < 0.05）。在基质胶上生长的系膜细胞也显示超氧化物歧化酶（SOD）的表达增强。我们得出结论，系膜细胞需要附着于基质才能存活，基质质量的改变调节系膜细胞凋亡。
