从粗制α淀粉酶制备的S1核酸酶中去除双链核酸酶活性。
Elimination of double strand nuclease activity from S1 nuclease prepared from crude alpha amylase.
作者信息
Hahn W E, Van Ness J
出版信息
Nucleic Acids Res. 1976 May;3(5):1419-23. doi: 10.1093/nar/3.5.1419.
Abstract
Single strand-specific s1 nuclease prepared as previously described from crude alpha amylase by DEAE-cellulose chromatography also contains nuclease which degrades double strand nucleic acid. The double strand activity can be removed by repeating the DEAE-cellulose chromatography procedure at least two additional times. S1 nuclease prepared by this procedure does not degrade double strand sheared DNA as measured by Sephadex chromatography. Under the same conditions single strand DNA is completely degraded. Thus, S1 nuclease prepared by this procedure is suitable for use in removing single strand regions in DNA/DNA duplexes and DNA/RNA hybrids.
摘要
如前所述，通过DEAE-纤维素色谱法从粗制α淀粉酶中制备的单链特异性s1核酸酶也含有可降解双链核酸的核酸酶。双链活性可以通过至少再重复两次DEAE-纤维素色谱法程序来去除。通过该程序制备的S1核酸酶经葡聚糖凝胶色谱法测定不会降解双链剪切DNA。在相同条件下，单链DNA会被完全降解。因此，通过该程序制备的S1核酸酶适用于去除DNA/DNA双链体和DNA/RNA杂交体中的单链区域。
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