通过聚合酶链反应对儿童B系急性淋巴细胞白血病进行克隆性研究。

Childhood B lineage acute lymphoblastic leukemia clonality study by the polymerase chain reaction.

作者信息

Scrideli C A, Simoes A L, Defavery R, Bernardes J E, Duarte M H, Tone L G

机构信息

Department of Puericulture and Pediatrics, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Brazil.

出版信息

J Pediatr Hematol Oncol. 1997 Nov-Dec;19(6):516-22. doi: 10.1097/00043426-199711000-00005.

DOI:10.1097/00043426-199711000-00005

PMID:9407937

Abstract

PURPOSE

B cell precursors acute lymphoblastic leukemia (ALL) present rearrangements in the heavy chain immunoglobulin and T cell receptor genes, especially in the complementarity determining region 3 (CDR-3) and T cell receptor delta (TCR delta) (V delta 2 D delta 3) regions. These rearrangements may be amplified by the polymerase chain reaction (PCR) and used as clonal markers of B lineage ALL. Our purpose was to study clonality at the DNA level by PCR in B lineage ALL.

PATIENTS AND METHODS

Fifty-three pediatric patients (36 with B lineage ALL, 7 with ALL-T, and 10 with nonlymphocytic disease) were investigated using consensus primers for the CDR-3 regions of IgH and TCR delta.

RESULTS

Clonality was detected in 86.1% of the patients with B lineage ALL when the primers for the CDR-3 regions were used, in 41.6% when the primers for TCR delta were used, and in 91.6% when the two primers were used together. Biclonality was found in 22.5% and 6.6% of patients that have shown clonality for CDR-3 and TCR delta, respectively. Clonality was not detected in any other samples using these primers.

CONCLUSIONS

PCR using CDR-3 and TCR delta primers can be used as an aid for B lineage ALL diagnosis and clonal evolution of theses disease.

摘要

目的

B 细胞前体急性淋巴细胞白血病（ALL）存在重链免疫球蛋白和 T 细胞受体基因重排，尤其是在互补决定区 3（CDR-3）和 T 细胞受体δ（TCRδ）（Vδ2 Dδ3）区域。这些重排可通过聚合酶链反应（PCR）扩增，并用作 B 系 ALL 的克隆标志物。我们的目的是通过 PCR 研究 B 系 ALL 的 DNA 水平克隆性。

患者和方法

使用针对 IgH 和 TCRδ的 CDR-3 区域的共有引物，对 53 例儿科患者（36 例 B 系 ALL、7 例 T 系 ALL 和 10 例非淋巴细胞疾病患者）进行了研究。

结果

当使用 CDR-3 区域引物时，86.1%的 B 系 ALL 患者检测到克隆性；使用 TCRδ引物时，41.6%的患者检测到克隆性；当两种引物一起使用时，91.6%的患者检测到克隆性。在分别显示 CDR-3 和 TCRδ克隆性的患者中，双克隆性分别在 22.5%和 6.6%的患者中发现。使用这些引物在任何其他样本中均未检测到克隆性。