Januszkiewicz D, Nowak J
Institute of Human Genetics Polish Academy of Sciences, Poznań.
Acta Haematol Pol. 1994;25(3):277-81.
DNA-based PCR with various sets of primers for T-cell receptor gamma/delta (TCR gamma/delta) chain genes was used to study clonality in childhood B-lineage acute lymphoblastic leukaemia. TCR delta genes rearrangements were the most common and were observed in 77 patients (64.2%). The typical pattern of rearrangements was defined as an incomplete V delta 2 to D delta 3 or to D delta 2 recombination product. Rearrangements of TCR gamma genes were observed in 61 cases (50.8%). Predominantly, TCR gamma genes rearrangements were detected in null-ALL and the early B-ALL (55.2% and 60%, respectively) and were rather rare in other groups. From all eight V segments of V gamma l group rearrangements concerned mostly regions V gamma 2, V gamma 4 and V gamma 7. We have confirmed that TCR gamma and delta genes amplification provides a rapid, sensitive method for assessing clonality in ALL almost in 100%.
采用基于DNA的聚合酶链反应（PCR）及多组针对T细胞受体γ/δ（TCRγ/δ）链基因的引物，研究儿童B系急性淋巴细胞白血病的克隆性。TCRδ基因重排最为常见，77例患者（64.2%）出现该情况。重排的典型模式定义为不完全的Vδ2至Dδ3或至Dδ2重组产物。61例（50.8%）患者出现TCRγ基因重排。主要在无丙种球蛋白血症性急性淋巴细胞白血病（null-ALL）和早期B系急性淋巴细胞白血病（early B-ALL）中检测到TCRγ基因重排（分别为55.2%和60%），在其他组中则较为罕见。Vγ1组的所有八个V区段重排大多涉及Vγ2、Vγ4和Vγ7区域。我们已证实，TCRγ和δ基因扩增为评估几乎100%的急性淋巴细胞白血病克隆性提供了一种快速、灵敏的方法。
