Jánossy T, Vizler C, Ocsovszki I, Tibbe G J, Pipis J, Savelkoul H F, Végh P, Benner R
Department of Experimental Surgery, Albert Szent-Györgyi Medical University, Hungary.
Acta Chir Hung. 1997;36(1-4):150-1.
In A/J (H-2a) (A) mice, the neonatal i.v. injection of (B10 x A)F1 spleen cells (SC) induces partial transplantation tolerance (TT) to C57BL/10ScSn (H-2b) (B10) skin allografts, chronic host-versus-graft disease (HVGD) and lethal lymphoproliferative disorders (LPD). They produce anti-T-cell autoantibodies (ATA), and the proliferative responses of their SC to the T cell mitogen Con A are decreased. We found that, similar to ATA, the hyporeactivity of T cells developed earlier (at 1-2 weeks of age) than splenomegaly. The proportions of both CD4+ and CD8+ T cells were not reduced in the spleens of tolerized mice without manifest LPD. The supernatants (SN) of Con A-stimulated tolerized SC contained no, or only small amounts of interleukin-2 (IL-2). Thus, in the tolerized mice, ATA and T cell deficiency preceded the development of LPD. ATA and the decreased amount of the T cell growth factor IL-2 might play a role in the defective T cell activation.
在A/J（H-2a）（A）小鼠中，新生期经静脉注射（B10×A）F1脾细胞（SC）可诱导对C57BL/10ScSn（H-2b）（B10）皮肤同种异体移植物产生部分移植耐受（TT）、慢性宿主抗移植物病（HVGD）和致死性淋巴细胞增殖性疾病（LPD）。它们会产生抗T细胞自身抗体（ATA），并且其脾细胞对T细胞有丝分裂原刀豆蛋白A（Con A）的增殖反应降低。我们发现，与ATA相似，T细胞的低反应性比脾肿大出现得更早（在1至2周龄时）。在没有明显LPD的耐受小鼠脾脏中，CD4⁺和CD8⁺ T细胞的比例并未降低。经Con A刺激的耐受脾细胞的上清液（SN）中不含或仅含有少量白细胞介素-2（IL-2）。因此，在耐受小鼠中，ATA和T细胞缺陷先于LPD的发生。ATA和T细胞生长因子IL-2量的减少可能在T细胞活化缺陷中起作用。
