Shetty G, Krishnamurthy H, Krishnamurthy H N, Ramachandra S G, Moudgal N R
Center for Reproductive Biology and Molecular Endocrinology, Indian Institute of Science, Bangalore, India.
Contraception. 1997 Oct;56(4):257-65. doi: 10.1016/s0010-7824(97)00136-4.
Administration of norethisterone (NET) or NET + estradiol benzoate using an Alzet minipump or as once-a-month intramuscular injection of their depot forms, NET-enanthate (NET-EN) and estradiol valerate (E-val), resulted in azoospermia in all monkeys (n = 13) within 60 to 150 days of treatment. Although addition of depot form of testosterone (T, 20 mg/month) to the regimen restored the behavioral response typical of a normal male, it did not reverse the azoospermic state. Serum T (heightened nocturnal) levels were significantly reduced (> 85%, p < 0.001) in all the treated groups. Evidence for blockade in spermatogenesis following treatment was obtained by DNA flow cytometry. Following withdrawal of treatment, the T level was restored to normalcy within 15 days but 120 days more were required for the animals to exhibit normal sperm counts. In conclusion, the efficacy of once-a-month injection of relatively low doses of NET-EN + E-Val to bring about azoospermia in monkeys, in a relatively short time, has been demonstrated. As the results are uniform and reproducible, it appears desirable that this steroid regimen be tested in man for its contraceptive efficacy.
使用Alzet微型泵或以每月一次肌肉注射其长效剂型炔诺酮庚酸酯（NET-EN）和戊酸雌二醇（E-val）的方式给予炔诺酮（NET）或NET +苯甲酸雌二醇，在治疗60至150天内导致所有猴子（n = 13）出现无精子症。尽管在该方案中添加长效睾酮（T，20mg/月）可恢复正常男性典型的行为反应，但并未逆转无精子状态。所有治疗组的血清T（夜间升高）水平均显著降低（> 85%，p < 0.001）。通过DNA流式细胞术获得了治疗后精子发生受阻的证据。停止治疗后，T水平在15天内恢复正常，但动物需要额外120天才能表现出正常的精子计数。总之，已证明每月一次注射相对低剂量的NET-EN + E-Val在相对较短的时间内可使猴子出现无精子症。由于结果一致且可重复，似乎有必要在人体中测试这种类固醇方案的避孕效果。
