采用高效液相色谱-紫外检测法测定人血浆中氯氮平及其代谢物N-去甲基氯氮平的含量。
Assay for quantitation of clozapine and its metabolite N-desmethylclozapine in human plasma by high-performance liquid chromatography with ultraviolet detection.
作者信息
Edno L, Combourieu I, Cazenave M, Tignol J
机构信息
Laboratoire de Biologie, Centre Hospitalier Charles Perrens, Bordeaux, France.
出版信息
J Pharm Biomed Anal. 1997 Oct;16(2):311-8. doi: 10.1016/s0731-7085(97)00048-4.
A high-performance liquid chromatographic method with UV detection has been developed for the analysis of clozapine and its active N-desmethylated metabolite (N-desmethylclozapine = DMC) in human plasma. A liquid/liquid procedure was used to extract clozapine and DMC from human plasma. The analysis was performed on a C8 Nucleosil column and the mobile phase comprised acetonitrile-water-Pic B5 diethylamine (63:37:25:0.04, v/v/v/v). The detection wavelength was 245 nm. The intra-assay and inter-assay precision was satisfactory within the concentration range 10-900 ng ml-1. The lower detection limit for clozapine and for DMC was 5 ng ml-1. The recovery and reproducibility values of this method were better or similar to those found by other authors. This method, which is simple, selective and avoids an evaporation step, can be used routinely for therapeutic drug monitoring.
已开发出一种带紫外检测的高效液相色谱法，用于分析人血浆中的氯氮平及其活性 N - 去甲基代谢物（N - 去甲基氯氮平 = DMC）。采用液 - 液萃取法从人血浆中提取氯氮平和 DMC。分析在 C8 核硅柱上进行，流动相由乙腈 - 水 - 匹克 B5 二乙胺（63:37:25:0.04，v/v/v/v）组成。检测波长为 245 nm。在 10 - 900 ng/ml 的浓度范围内，批内和批间精密度令人满意。氯氮平和 DMC 的最低检测限为 5 ng/ml。该方法的回收率和重现性值优于或类似于其他作者所报道的值。此方法简单、具有选择性且无需蒸发步骤，可常规用于治疗药物监测。
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