Jáuregui O, Moyano E, Galceran M T
Departament de Química Analítica, Universitat de Barcelona, Spain.
J Chromatogr A. 1997 Nov 7;787(1-2):79-89. doi: 10.1016/s0021-9673(97)00653-5.
Liquid chromatography coupled to atmospheric-pressure ionization mass spectrometry (LC-API-MS) with negative ion detection was studied for the determination of a variety of phenolic compounds in environmental waters. An isocratic mobile phase of 0.05% acetic acid-acetonitrile (50:50, v/v) was used. The influence of post-column addition of different bases on the sensitivity of the detection in electrospray (ES) was studied. The [M-H]-ion was the base peak for all the compounds using both ES and atmospheric-pressure chemical-ionization (APCI) ion sources. Moreover, abundant structural information was obtained by increasing the extraction voltage. Detection limits for standard solutions ranging from 2 to 13 ng injected for LC-ES-MS and from 0.02 to 20 ng for LC-APCI-MS were obtained. Good reproducibilities (day-to-day and run-to-run) were observed. The optimum LC-ES-MS and LC-APCI-MS conditions thus determined were used for a quantitative analysis of some phenolic compounds in spiked tap water and sea water samples.
研究了采用负离子检测的液相色谱-大气压电离质谱联用技术（LC-API-MS）测定环境水体中多种酚类化合物。使用了0.05%乙酸-乙腈（50:50，v/v）的等度流动相。研究了柱后添加不同碱对电喷雾（ES）检测灵敏度的影响。使用ES和大气压化学电离（APCI）离子源时，[M-H]-离子是所有化合物的基峰。此外，通过提高提取电压获得了丰富的结构信息。对于LC-ES-MS，进样2至13 ng标准溶液的检测限，对于LC-APCI-MS，进样0.02至20 ng标准溶液的检测限均已得到。观察到良好的重现性（日间和批间）。由此确定的最佳LC-ES-MS和LC-APCI-MS条件用于加标自来水和海水样品中某些酚类化合物的定量分析。
