通过尺寸排阻色谱结合小角度光散射光度法和粘度测定法对抗抗原抗体复合物进行表征。

Characterization of antigen-antibody complexes by size-exclusion chromatography coupled with low-angle light-scattering photometry and viscometry.

作者信息

Qian R L, Mhatre R, Krull I S

机构信息

Department of Chemistry, Northeastern University, Boston, MA 02115, USA.

出版信息

J Chromatogr A. 1997 Nov 7;787(1-2):101-9. doi: 10.1016/s0021-9673(97)00666-3.

DOI:10.1016/s0021-9673(97)00666-3

PMID:9408997

Abstract

In this paper, the molecular masses (M(r)s) of the complexes of monoclonal anti-BSA (antibody to bovine serum albumin) (clone: 33) and monomer BSA were determined on-line by using size-exclusion chromatography (SEC) coupled with a low-angle laser light-scattering (LALLS) detector and two concentration detectors, ultraviolet (UV) and refractive index (RI) (SEC-LALLS/UV/RI system). Also, the size and M(r)s of the complexes were evaluated by the SEC-LALLS/UV/viscometer (VISC) system. This study demonstrated that, for small size macromolecules, the combination of light scattering and viscosity detection was a suitable choice for determining their M(r)s and sizes.

摘要

在本文中，使用尺寸排阻色谱法（SEC）结合低角度激光光散射（LALLS）检测器以及两个浓度检测器，即紫外（UV）和折光指数（RI）（SEC-LALLS/UV/RI系统），在线测定了单克隆抗牛血清白蛋白（BSA）（抗体克隆：33）与单体BSA复合物的分子量（M(r)s）。此外，通过SEC-LALLS/UV/粘度计（VISC）系统评估了复合物的大小和M(r)s。本研究表明，对于小尺寸大分子，光散射和粘度检测相结合是测定其M(r)s和大小的合适选择。