Toida T, Shima M, Azumaya S, Maruyama T, Toyoda H, Imanari T, Linhardt R J
Faculty of Pharmaceutical Sciences, Chiba University, Japan.
J Chromatogr A. 1997 Nov 7;787(1-2):266-70. doi: 10.1016/s0021-9673(97)00658-4.
Glycosaminoglycans including heparin, heparan sulfate, chondroitin sulfate, dermatan sulfate and hyaluronan were analyzed by high-performance gel filtration chromatography. Detection was achieved at 240 nm based on the formation of copper(II) complex in copper sulfate solution at low pH. Detection of the copper(II)-heparin complex is sensitive, permitting the analysis of as little as 10(-7) g. This method was also successfully applied to the analysis of the chemically derivatized glycosaminoglycans (de-N-acetylated and/or oversulfated chondroitin sulfate) that cannot be depolymerized by enzymes such as heparin and chondroitin lyases.
通过高效凝胶过滤色谱法分析了包括肝素、硫酸乙酰肝素、硫酸软骨素、硫酸皮肤素和透明质酸在内的糖胺聚糖。在低pH值的硫酸铜溶液中，基于铜（II）络合物的形成，于240nm处进行检测。铜（II）-肝素络合物的检测灵敏度高，能够分析低至10^(-7)g的样品。该方法还成功应用于化学衍生糖胺聚糖（脱N-乙酰化和/或过度硫酸化硫酸软骨素）的分析，这些糖胺聚糖不能被肝素酶和软骨素裂解酶等酶解聚。
