钙及受体对大鼠腮腺腺泡分散细胞放射性钠摄取的调节
Calcium and receptor regulation of radiosodium uptake by dispersed rat parotid acinar cells.
作者信息
Landis C A, Putney J W
出版信息
J Physiol. 1979 Dec;297(0):369-77. doi: 10.1113/jphysiol.1979.sp013045.
Abstract
- Dispersed rat parotid acinar cells were used to study the effects of secretagogues on 22Na uptake. 2. Carbachol stimulated 22Na uptake, and caused a net gain in total Na and a loss in total K. These effects were accentuated in the presence of 10(-3) M-ouabain. 3. Substance P, epinephrine and phenylephrine also stimulated 22Na uptake while isoproterenol and angiotensin II did not. 4. The 22Na uptake due to carbachol was inhibited by atropine, procaine or CoCl2; the response to Substance P was inhibited by CoCl2 only. 5. Extracellular Ca was required for stimulation of 22Na uptake by carbachol. Strontium but not Ba could substitute for Ca in supporting 22Na uptake. 6. Uptake of 22Na was stimulated by the divalent cationophore, A-23187, and Ca was required for this effect. 7. It is concluded that activation of Ca influx by muscarinic, alpha-adrenergic or peptide agonists triggers, among other effects, an increased membrane permeability to Na.
摘要
- 采用分散的大鼠腮腺腺泡细胞研究促分泌剂对22Na摄取的影响。2. 卡巴胆碱刺激22Na摄取，并导致总钠净增加和总钾减少。在存在10(-3) M哇巴因的情况下，这些效应会增强。3. P物质、肾上腺素和去氧肾上腺素也刺激22Na摄取，而异丙肾上腺素和血管紧张素II则无此作用。4. 卡巴胆碱引起的22Na摄取受到阿托品、普鲁卡因或CoCl2的抑制；对P物质的反应仅受到CoCl2的抑制。5. 细胞外钙是卡巴胆碱刺激22Na摄取所必需的。锶可替代钙支持22Na摄取，而钡则不能。6. 二价阳离子载体A-23187刺激22Na摄取，且此效应需要钙。7. 得出结论：毒蕈碱、α-肾上腺素能或肽类激动剂激活钙内流除其他作用外，还会引发膜对钠的通透性增加。
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引用本文的文献
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Influence of pilocarpine on transport by salivary gland slices.毛果芸香碱对唾液腺切片转运的影响。
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