Cho M S, Lee M K, Yang W I, Yoon J H
Department of Pathology, Yonsei University College of Medicine, Seoul, Korea.
Yonsei Med J. 1997 Oct;38(5):285-93. doi: 10.3349/ymj.1997.38.5.285.
Granzyme B is one of the serine proteases expressed in natural killer (NK) cells and activated cytotoxic T-lymphocytes. To evaluate the usefulness of granzyme B immunoreactivity in the diagnosis of T/NK-cell lymphoma, we studied 69 cases of lymphomas occurring in the upper aerodigestive tract by paraffin-section immunohistochemistry using a commercially available monoclonal antibody to granzyme B (GrB-7). All 19 cases of T/NK-cell lymphomas defined by the expression of CD56 (NHK-1) and one or both T-cell markers (polyclonal CD3 and CD45RO) expressed granular cytoplasmic granzyme B immunoreactivity. Two out of 9 cases of T-cell lymphomas showing no CD56 expression demonstrated strong granzyme B immunoreactivity. No tumor cells among 39 cases of B-cell diffuse large cell lymphomas and 2 cases of null cell diffuse large cell lymphomas were immunoreactive for granzyme B, however a few scattered granzyme B-positive reactive small lymphoid cells were consistently observed. Based on its sensitivity in this study as well as its reactivity in routinely processed tissue sections, even without heat-induced epitope retrieval technique, monoclonal antibody to granzyme B (GrB-7) can be applied as a useful marker in the diagnosis of T/NK-cell lymphomas in conjunction with CD56.
颗粒酶B是自然杀伤（NK）细胞和活化的细胞毒性T淋巴细胞中表达的丝氨酸蛋白酶之一。为了评估颗粒酶B免疫反应性在T/NK细胞淋巴瘤诊断中的作用，我们使用市售的抗颗粒酶B单克隆抗体（GrB-7），通过石蜡切片免疫组织化学研究了69例发生在上呼吸道消化道的淋巴瘤。所有19例由CD56（NHK-1）和一种或两种T细胞标志物（多克隆CD3和CD45RO）表达所定义的T/NK细胞淋巴瘤均表现出颗粒状细胞质颗粒酶B免疫反应性。9例无CD56表达的T细胞淋巴瘤中有2例表现出强颗粒酶B免疫反应性。39例B细胞弥漫性大细胞淋巴瘤和2例无细胞弥漫性大细胞淋巴瘤的肿瘤细胞均无颗粒酶B免疫反应性，然而，始终观察到少数散在的颗粒酶B阳性反应性小淋巴细胞。基于其在本研究中的敏感性以及在常规处理的组织切片中的反应性，即使不使用热诱导抗原修复技术，抗颗粒酶B单克隆抗体（GrB-7）也可与CD56联合用作T/NK细胞淋巴瘤诊断中的有用标志物。
