Takayama S, Murakami S, Miki Y, Ikezawa K, Tasaka S, Terashima A, Asano T, Okada H
Department of Periodontology and Endodontology, Osaka University Faculty of Dentistry, Japan.
J Periodontal Res. 1997 Nov;32(8):667-75. doi: 10.1111/j.1600-0765.1997.tb00577.x.
In order to clarify the regulatory mechanisms of periodontal regeneration by basic fibroblast growth factor (bFGF), effects of bFGF on proliferation, alkaline phosphatase activity, calcified nodule formation and extracellular matrix synthesis of human periodontal ligament (PDL) cells were examined in this study. bFGF enhanced the proliferative responses of PDL cells in a dose-dependent manner. The maximum mitogenic effect of bFGF on PDL cells was observed at the concentration of 10 ng/ml. In contrast, bFGF inhibited the induction of alkaline phosphatase activity and the mineralized nodule formation by PDL cells. Moreover, employing the reverse transcription-polymerase chain reaction (RT-PCR) technique, we observed that the levels of laminin mRNA of human PDL cells was specifically upregulated by bFGF stimulation, but that of type I collagen mRNA was downregulated. On the other hand, the expression of type III collagen and fibronectin mRNA were not altered even when the cells were activated by bFGF. These results suggest that suppressing cytodifferentiation of PDL cells into mineralized tissue forming cells, bFGF may play a role in wound healing by inducing growth of immature PDL cells and that in turn accelerates periodontal regeneration.
为阐明碱性成纤维细胞生长因子（bFGF）对牙周组织再生的调控机制，本研究检测了bFGF对人牙周膜（PDL）细胞增殖、碱性磷酸酶活性、钙化结节形成及细胞外基质合成的影响。bFGF以剂量依赖方式增强PDL细胞的增殖反应。在10 ng/ml浓度时观察到bFGF对PDL细胞的最大促有丝分裂作用。相反，bFGF抑制PDL细胞碱性磷酸酶活性的诱导及矿化结节形成。此外，采用逆转录-聚合酶链反应（RT-PCR）技术，我们观察到bFGF刺激可使人类PDL细胞层粘连蛋白mRNA水平特异性上调，但I型胶原mRNA水平下调。另一方面，即使细胞被bFGF激活，III型胶原和纤连蛋白mRNA的表达也未改变。这些结果表明，bFGF通过抑制PDL细胞向矿化组织形成细胞的细胞分化，可能在诱导未成熟PDL细胞生长从而促进伤口愈合中发挥作用，进而加速牙周组织再生。
