牛肝脏中肉碱棕榈酰转移酶的活性

Hepatic carnitine palmitoyltransferase activity in cattle.

作者信息

Mizutani H, Sako T, Takemura N, Koyama H, Yamaguchi M, Motoyoshi S

机构信息

Department of Veterinary Internal Medicine, Nippon Veterinary and Animal Science University, Tokyo, Japan.

出版信息

J Vet Med Sci. 1997 Nov;59(11):1067-9. doi: 10.1292/jvms.59.1067.

DOI:10.1292/jvms.59.1067

PMID:9409528

Abstract

A fluorometric assay for the determination of hepatic carnitine palmitoyltransferase (CPT) activity was slightly modified for use with cattle samples. With this assay, the Km value was 0.56 +/- 0.10 mM with respect to L-carnitine (mean +/- SD, n = 4) and was 9.6 +/- 2.2 microM (n = 3) with respect to palmitoyl-CoA. The average hepatic CPT activity was 33.6 +/- 2.0 mumol CoASH/min/g protein in 38 healthy cattle and was similar in both sexes and among breeds. Hepatic CPT activity showed no correlation with serum phospholipid, free fatty acid, triglyceride or total cholesterol concentrations.

摘要

一种用于测定肝脏肉碱棕榈酰转移酶（CPT）活性的荧光测定法稍作修改后用于牛样本。通过该测定法，相对于L-肉碱，Km值为0.56±0.10 mM（平均值±标准差，n = 4），相对于棕榈酰辅酶A，Km值为9.6±2.2 μM（n = 3）。38头健康牛的肝脏CPT平均活性为33.6±2.0 μmol辅酶A/分钟/克蛋白质，且在性别和品种间相似。肝脏CPT活性与血清磷脂、游离脂肪酸、甘油三酯或总胆固醇浓度均无相关性。