Loftenius A, Ekstrand J, Möller E
Department of Basic Oral Sciences, Karolinska Institute, Huddinge, Sweden.
Clin Exp Immunol. 1997 Dec;110(3):418-22. doi: 10.1046/j.1365-2249.1997.4531470.x.
Due to the release of the toxic compounds of mercury from amalgam fillings, dental amalgam has been questioned as an adequate restoration material for tooth fillings. HgCl2 has been found to be mitogenic for human blood lymphocytes in vitro. However, activation required much higher concentrations than are ever found in vivo. This study has been initiated to evaluate further the influence of HgCl2 on human immunocompetent cells in vitro. It is found that HgCl2 in a narrow concentration range has the ability to preferentially stimulate the CD4+ T cell subset to blast transformation and DNA synthesis. The reaction, when monitored during days 2-6, is maximal at day 6, and most blasts express the IL-2 receptor (IL-2R), indicating in vitro activation. The CD8+ T cell subset is not affected to the same extent. In addition, HgCl2-induced lymphocyte reactivity is dependent on accessory cells, i.e. CD14+ cells.
由于汞合金填充物会释放有毒汞化合物，牙科汞合金作为一种合适的牙齿填充修复材料受到了质疑。已发现HgCl2在体外对人血淋巴细胞具有促有丝分裂作用。然而，激活所需的浓度比体内发现的浓度要高得多。启动本研究以进一步评估HgCl2在体外对人免疫活性细胞的影响。结果发现，在狭窄浓度范围内的HgCl2能够优先刺激CD4 + T细胞亚群发生母细胞转化和DNA合成。在第2 - 6天监测该反应时，第6天反应最大，大多数母细胞表达IL - 2受体（IL - 2R），表明发生了体外激活。CD8 + T细胞亚群受到的影响程度不同。此外，HgCl2诱导的淋巴细胞反应性依赖于辅助细胞即CD14 +细胞。
