von Weikersthal S F, Seetharaman S, Wright K A, Roux F A, Barrand M A
Department of Pharmacology, University of Cambridge, UK.
FEBS Lett. 1997 Nov 17;417(3):395-9. doi: 10.1016/s0014-5793(97)01320-3.
Hypotonicity-induced anion permeability changes were investigated but not detected in immortalised (RBE4) rat brain endothelial cells using iodide efflux measurements. Large, rapid increases were however observed in primary cultured cells. Both cell types were reinvestigated following culture in a common growth factor-depleted medium. Responses were still undetectable in the immortalised RBE4 cells. Reduced responses were observed in the primary cultured cells that also showed altered morphology and decreased activity of another transporter, P-glycoprotein. Thus both immortalisation and different culture conditions may alter functional expression in these cells of transporters involved in hypotonicity-induced anion permeability changes.
利用碘外流测量法对低渗诱导的阴离子通透性变化进行了研究，但在永生化（RBE4）大鼠脑内皮细胞中未检测到这种变化。然而，在原代培养细胞中观察到了大幅度的快速增加。在共同的缺乏生长因子的培养基中培养后，对这两种细胞类型都进行了重新研究。在永生化RBE4细胞中仍未检测到反应。在原代培养细胞中观察到反应减弱，这些细胞还表现出形态改变以及另一种转运蛋白P-糖蛋白的活性降低。因此，永生化和不同的培养条件都可能改变这些细胞中参与低渗诱导的阴离子通透性变化的转运蛋白的功能表达。
