Kerstein M D, Tonkens R M
Surg Gynecol Obstet. 1976 Aug;143(2):253-6.
The effectiveness of an elemental diet was investigated as both a prophylactic and therapeutic agent in experimental canine pancreatitis. Pancreatitis was induced by operative injection of a bile -saline solution mixture under pressure retrograde into the main pancreatic duct. In addition to a preinjection control sample, serial biopsies were obtained at 30 minute intervals for 90 minutes after injection and fixed for light and electron microscopic examinations. In addition, preoperative and postoperative blood samples were drawn and analyzed for amylase. After operation, half of the dogs from each original group were fed Vivonex-100, the other half from each group, regular laboratory chow, yielding four ultimate groups based on preoperative and postoperative diets. Successful induction of pancreatitis was evaluated by the difference between preoperative and postoperative amylase values, all of which were significant by group at the p less than 0.01 level. No ultrastructural evidence was found for the modification of zymogen granules with the pretreatment elemental diet nor were differences evident, histologically or ultrastructurally, in the severity of pancreatitis between the pretreated and nonpretreated groups. Finally, gross mortality figures demonstrated no efficacy of elemental diet for pretreatment prophylaxis of acute pancreatitis.
研究了要素饮食作为实验性犬胰腺炎预防和治疗药物的有效性。通过在压力下将胆盐溶液混合物逆行手术注入主胰管来诱发胰腺炎。除了注射前的对照样本外，在注射后90分钟内每隔30分钟进行一次连续活检，并固定用于光镜和电镜检查。此外，采集术前和术后血样并分析淀粉酶。手术后，每组原分组中的一半犬只喂食Vivonex - 100，每组另一半喂食常规实验室饲料，根据术前和术后饮食产生四个最终分组。通过术前和术后淀粉酶值的差异评估胰腺炎的成功诱发情况，所有组在p小于0.01水平时差异均具有统计学意义。预处理要素饮食未发现对酶原颗粒有超微结构改变的证据，预处理组和未预处理组在胰腺炎严重程度方面，在组织学或超微结构上也没有明显差异。最后，总死亡率数据表明要素饮食对急性胰腺炎的预处理预防无效。
