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采用缓释形式的蜕皮激素成功诱导成年雄性美洲龙虾(美洲螯龙虾)蜕皮。

The successful induction of molting in the adult male lobster (Homarus americanus) with a slow-release form of ecdysterone.

作者信息

Gilgan M W, Burns B G

出版信息

Steroids. 1976 May;27(5):571-80. doi: 10.1016/s0039-128x(76)90048-9.

Abstract

In an initial, and then a confirmatory experiment, adult, male lobsters were injected with solvent, ecdysterone (E, 2.0 mug/g live weight) or ecdysterone acetate (EAc, 2.5 or 5.0 mug/g live weight) emulsions in Freund's incomplete adjuvant (FIA). Control lobsters underwent no molts and only one death in the two cases. The E treated animals all died (average: exp. 1, 19.2 +/-2.1 days; exp. 2, 25.3 +/- 8 days). After the lobsters were treated twice with 2.5 mug EAc/g live weight, in the first experiment, four out of five molted and one died; in the second experiment six out of eight molted, one died and one remained refractory. The high EAc dose resulted in five deaths, one molt and two pseudomolts after one treatment. It is concluded that the use of the oil emulsion and EAc sufficiently slowed the release of free ecdysterone to allow complete premolt development in the lobster.

摘要

在一项初始实验以及随后的验证性实验中,成年雄性龙虾被注射了溶剂、蜕皮激素(E,2.0微克/克活体重)或醋酸蜕皮激素(EAc,2.5或5.0微克/克活体重)与弗氏不完全佐剂(FIA)的乳剂。对照龙虾没有蜕皮,在这两个案例中仅出现一例死亡。接受E处理的动物全部死亡(平均:实验1,19.2±2.1天;实验2,25.3±8天)。在用2.5微克EAc/克活体重对龙虾进行两次处理后,在第一个实验中,五只中有四只蜕皮,一只死亡;在第二个实验中,八只中有六只蜕皮,一只死亡,一只仍未出现反应。高剂量EAc处理一次后导致五只死亡、一只蜕皮和两次假蜕皮。得出的结论是,油乳剂和EAc的使用充分减缓了游离蜕皮激素的释放,从而使龙虾能够完成蜕皮前的完全发育。

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