Potoki L V
Tsitologiia. 1976 Jan;18(1):10-5.
The responses of interphase and mitotic chromatins to beta-mercaptoethanol were studied. The reaction of X-chromatin of interphase nuclei was expressed in the increase of the percentage of cells with X-chromatin. beta-Merceptoethanol removed a condensing effect induced by spermine on the diffused chromatin of interphase cultured fibroblasts. beta-Mercaptoethanol caused a differential staining of metaphase chromosome when added to the cultured cells 0.5 hours prior to fixation. The treatment of chromosome preparations with different concentrations of beta-merceptoethanol and for different times led to different staining of chromosomes with asur--eosin. Low concentrations and/or short treatment induced G-banding, most typical with moderate concentrations. Higher concentrations and longer treatment prevented G-staining due to the loosening of G-segments.
研究了间期染色质和有丝分裂染色质对β-巯基乙醇的反应。间期核X染色质的反应表现为具有X染色质的细胞百分比增加。β-巯基乙醇消除了精胺对间期培养成纤维细胞弥散染色质的凝聚作用。在固定前0.5小时将β-巯基乙醇加入培养细胞时，可使中期染色体产生差异染色。用不同浓度的β-巯基乙醇处理染色体标本并持续不同时间，会导致染色体用苏木精-伊红染色呈现不同结果。低浓度和/或短时间处理诱导G带形成，中等浓度时最为典型。较高浓度和较长时间处理由于G段松解而阻止了G染色。
