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[使用标准通过细胞光度法对酶活性进行定量评估]

[A quantitative assessment of enzyme activity by cytophotometry using a standard].

作者信息

Anders V N, Sal'nikov V V

出版信息

Tsitologiia. 1976;18(5):636-8.

PMID:941296
Abstract

The usage of the standard has been offered in cytospectrophotometrical measurements of enzymatic activities that are revealed histochemically. A tetrazolium method was used to demonstrate lactate dehydrogenase activity in sections of the rat brain. As a standard, a mixture of the enzyme (a crystalline forme of LDG) and gelatine was taken. The method of standardization permits fulfilling a quantitative comparison of heterogenic biological materials.

摘要

该标准已应用于通过细胞分光光度法对组织化学显示的酶活性进行测量。采用四氮唑法在大鼠脑切片中显示乳酸脱氢酶活性。以酶(乳酸脱氢酶的结晶形式)和明胶的混合物作为标准品。标准化方法允许对异质生物材料进行定量比较。

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1
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Tsitologiia. 1976;18(5):636-8.
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