Ramírez M J, Surrallés J, Puerto S, Creus A, Marcos R
Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra (Cerdanyola del Vallès), Spain.
Mutagenesis. 1997 Nov;12(6):405-10. doi: 10.1093/mutage/12.6.405.
The effects induced by aneugenic agents on chromosome segregation are manifold. The biological relevance of these effects has led to the development of assays specifically detecting aneugens. In this context, the micronucleus (MN) assay in binucleated human lymphocytes along with FISH has been considered a pertinent tool for detecting aneugenic and clastogenic activity. However, the MN assay is insensitive in detecting aneugenic effects other than chromosome loss. By using the aneugenic model compound colchicine and X chromosome centromere-specific FISH, we have shown that besides chromosome loss in binucleated cells, other effects such as MN in mononucleated cells, cells arrested at metaphase, polyploidy and non-disjunction are also consistently induced by aneugenic agents. A chromosome 1 centromeric probe was used simultaneously with X chromosome centromeric labeling in mononucleated cells in order to distinguish polysomy from polyploidy. It is concluded that all these effects should be considered for a comprehensive evaluation of aneugenic activity.
非整倍体诱导剂对染色体分离的影响是多方面的。这些影响的生物学相关性促使了专门检测非整倍体的检测方法的发展。在此背景下，双核人淋巴细胞中的微核（MN）检测结合荧光原位杂交（FISH）被认为是检测非整倍体和致断裂活性的相关工具。然而，MN检测在检测除染色体丢失以外的非整倍体效应方面并不敏感。通过使用非整倍体模型化合物秋水仙碱和X染色体着丝粒特异性FISH，我们已经表明，除了双核细胞中的染色体丢失外，非整倍体诱导剂还会持续诱导其他效应，如单核细胞中的微核、停滞在中期的细胞、多倍体和不分离。在单核细胞中同时使用1号染色体着丝粒探针和X染色体着丝粒标记，以区分多体性和多倍体。结论是，为了全面评估非整倍体活性，应考虑所有这些效应。
